Abstract：
Sugar cane extract (SCE) is the end product of glucose, fructose, and sucrose elimination in molasses. SCE has various biological effects, such as anti-inflammation and antioxidation, and it is commonly found in animal feed. The present research is aimed at investigating the reproductive endocrine influence of SCE in male Japanese quails (Coturnix japonica) by feeding SCE containing food. In addition, in vitro Leydig cell culture was conducted to clarify the mechanism of SCE\'s influence. Our results showed that SCE feed extended the latency to the first neck grab, decreased male quail testis and epididymis weights, cloaca gland size, and reduced serum testosterone concentrations. Steroidogenic enzymes 3βHSD, 17βHSD, P450c17, and P450scc gene expression in the testis were decreased in the SCE groups. Western blot analysis showed decreased 3βHSD in the testis after feeding SCE. Isolated testicular interstitial cells cultured with SCE and ovine-LH suppressed testosterone secretion and 3βHSD gene expression. In conclusion, SCE as a feed additive has an impact on the sexual behavior and reproductive function of male Japanese quail, with the suppression of steroidogenesis in the Leydig cell. Our results may provide beneficial information to the livestock management and the poultry industry.
摘要：
甘蔗提取物（SCE）是葡萄糖的最终产物，果糖,和糖蜜中的蔗糖消除。SCE具有多种生物学效应，如抗炎和抗氧化，它常见于动物饲料中。本研究旨在通过饲喂含SCE的食物来研究SCE对雄性日本鹌鹑（Coturnixjaponica）的生殖内分泌影响。此外,进行了体外Leydig细胞培养以阐明SCE的影响机制。我们的结果表明，SCE饲料将潜伏期延长到第一次颈部抓取，雄性鹌鹑睾丸和附睾重量减少，泄殖腔腺体大小,降低血清睾酮浓度.类固醇生成酶3βHSD，17βHSD,SCE组睾丸中P450c17和P450scc基因表达均降低。Western印迹分析显示，饲喂SCE后睾丸中3βHSD降低。用SCE和绵羊-LH培养的分离的睾丸间质细胞抑制了睾丸激素的分泌和3βHSD基因的表达。总之,SCE作为饲料添加剂对雄性日本鹌鹑的性行为和生殖功能有影响，抑制睾丸间质细胞中的类固醇生成。我们的结果可能为畜牧业管理和家禽业提供有益的信息。
