PDF(Pubmed)
Abstract：
Tear proteomic changes can be a candidate etiopathogenesis of lacrimal duct obstruction diseases (LDODs). Studies on proteomics have focused primarily on nasolacrimal duct obstruction, and some specific inflammatory cytokines such as interferon (IFN)-α2a, interleukin (IL)-8 and IL-10, have not been investigated. In addition, differences in inflammatory cytokines in tears according to the LDOD subtype have not been reported. This study aimed to quantitatively compare inflammatory cytokines in tears from patients with LDOD and investigate tear-cytokine differences among different LDOD subtypes.
Tear samples were collected from both eyes of 30 patients with unilateral LDOD: five patients with prelacrimal obstruction, five with acute dacryocystitis and 20 with chronic dacryocystitis. The contralateral eyes were used as controls. IFN-α2a, IFN-β, IFN-γ, IL-17A, IL-6, IL-8, tumour necrosis factor-alpha (TNF-α), vascular endothelial growth factor (VEGF)-A, induced protein-10 (IP-10) and monocyte chemotactic protein-1 (MCP-1) were quantified in all samples.
The expression of eight cytokines (except for IP-10 and MCP-1) were significantly increased in the affected eyes compared with those in the control eyes. The levels of nine inflammatory cytokines (except for IP-10) in the affected eyes of patients with chronic dacryocystitis were higher than those in the affected eyes of patients with prelacrimal obstruction. In addition, patients with chronic dacryocystitis presented significantly higher IFN-γ level than those with prelacrimal obstruction or acute dacryocystitis.
Specific pro-inflammatory cytokines were increased in tears of patients with LDOD compared with those in the controls. The specific cytokine profiles observed in the tears of individuals with different LDOD subtypes may be associated with the unique aetiopathogenesis of these conditions.
Tear samples were collected from both eyes of 30 patients with unilateral LDOD: five patients with prelacrimal obstruction, five with acute dacryocystitis and 20 with chronic dacryocystitis. The contralateral eyes were used as controls. IFN-α2a, IFN-β, IFN-γ, IL-17A, IL-6, IL-8, tumour necrosis factor-alpha (TNF-α), vascular endothelial growth factor (VEGF)-A, induced protein-10 (IP-10) and monocyte chemotactic protein-1 (MCP-1) were quantified in all samples.
The expression of eight cytokines (except for IP-10 and MCP-1) were significantly increased in the affected eyes compared with those in the control eyes. The levels of nine inflammatory cytokines (except for IP-10) in the affected eyes of patients with chronic dacryocystitis were higher than those in the affected eyes of patients with prelacrimal obstruction. In addition, patients with chronic dacryocystitis presented significantly higher IFN-γ level than those with prelacrimal obstruction or acute dacryocystitis.
Specific pro-inflammatory cytokines were increased in tears of patients with LDOD compared with those in the controls. The specific cytokine profiles observed in the tears of individuals with different LDOD subtypes may be associated with the unique aetiopathogenesis of these conditions.
摘要：
泪液蛋白质组变化可能是泪管阻塞疾病（LDODs）的候选病因。蛋白质组学的研究主要集中在鼻泪管阻塞，和一些特定的炎症细胞因子，如干扰素（IFN）-α2a，白细胞介素（IL）-8和IL-10尚未被研究。此外,根据LDOD亚型，泪液中炎性细胞因子的差异尚未报道。本研究旨在定量比较LDOD患者泪液中的炎性细胞因子，并研究不同LDOD亚型之间的泪液细胞因子差异。
从30例单侧LDOD患者的双眼收集泪液样本：5例泪前阻塞患者，5例急性泪囊炎和20例慢性泪囊炎。对侧眼睛用作对照。IFN-α2a,IFN-β,IFN-γ，IL-17A,IL-6，IL-8，肿瘤坏死因子-α（TNF-α），血管内皮生长因子(VEGF)-A,对所有样品中的诱导蛋白-10(IP-10)和单核细胞趋化蛋白-1(MCP-1)进行定量.
与对照组相比，受影响的眼睛中八种细胞因子（IP-10和MCP-1除外）的表达显着增加。慢性泪囊炎患者患眼的9种炎性细胞因子(IP-10除外)水平高于泪前阻塞患者患眼。此外,慢性泪囊炎患者的IFN-γ水平明显高于泪前阻塞或急性泪囊炎患者.
与对照组相比，LDOD患者泪液中的特异性促炎细胞因子增加。在具有不同LDOD亚型的个体的泪液中观察到的特定细胞因子谱可能与这些病症的独特病因有关。
从30例单侧LDOD患者的双眼收集泪液样本：5例泪前阻塞患者，5例急性泪囊炎和20例慢性泪囊炎。对侧眼睛用作对照。IFN-α2a,IFN-β,IFN-γ，IL-17A,IL-6，IL-8，肿瘤坏死因子-α（TNF-α），血管内皮生长因子(VEGF)-A,对所有样品中的诱导蛋白-10(IP-10)和单核细胞趋化蛋白-1(MCP-1)进行定量.
与对照组相比，受影响的眼睛中八种细胞因子（IP-10和MCP-1除外）的表达显着增加。慢性泪囊炎患者患眼的9种炎性细胞因子(IP-10除外)水平高于泪前阻塞患者患眼。此外,慢性泪囊炎患者的IFN-γ水平明显高于泪前阻塞或急性泪囊炎患者.
与对照组相比，LDOD患者泪液中的特异性促炎细胞因子增加。在具有不同LDOD亚型的个体的泪液中观察到的特定细胞因子谱可能与这些病症的独特病因有关。
