Tear samples were collected from both eyes of 30 patients with unilateral LDOD: five patients with prelacrimal obstruction, five with acute dacryocystitis and 20 with chronic dacryocystitis. The contralateral eyes were used as controls. IFN-α2a, IFN-β, IFN-γ, IL-17A, IL-6, IL-8, tumour necrosis factor-alpha (TNF-α), vascular endothelial growth factor (VEGF)-A, induced protein-10 (IP-10) and monocyte chemotactic protein-1 (MCP-1) were quantified in all samples.
The expression of eight cytokines (except for IP-10 and MCP-1) were significantly increased in the affected eyes compared with those in the control eyes. The levels of nine inflammatory cytokines (except for IP-10) in the affected eyes of patients with chronic dacryocystitis were higher than those in the affected eyes of patients with prelacrimal obstruction. In addition, patients with chronic dacryocystitis presented significantly higher IFN-γ level than those with prelacrimal obstruction or acute dacryocystitis.
Specific pro-inflammatory cytokines were increased in tears of patients with LDOD compared with those in the controls. The specific cytokine profiles observed in the tears of individuals with different LDOD subtypes may be associated with the unique aetiopathogenesis of these conditions.
从30例单侧LDOD患者的双眼收集泪液样本:5例泪前阻塞患者,5例急性泪囊炎和20例慢性泪囊炎。对侧眼睛用作对照。IFN-α2a,IFN-β,IFN-γ,IL-17A,IL-6,IL-8,肿瘤坏死因子-α(TNF-α),血管内皮生长因子(VEGF)-A,对所有样品中的诱导蛋白-10(IP-10)和单核细胞趋化蛋白-1(MCP-1)进行定量.
与对照组相比,受影响的眼睛中八种细胞因子(IP-10和MCP-1除外)的表达显着增加。慢性泪囊炎患者患眼的9种炎性细胞因子(IP-10除外)水平高于泪前阻塞患者患眼。此外,慢性泪囊炎患者的IFN-γ水平明显高于泪前阻塞或急性泪囊炎患者.
与对照组相比,LDOD患者泪液中的特异性促炎细胞因子增加。在具有不同LDOD亚型的个体的泪液中观察到的特定细胞因子谱可能与这些病症的独特病因有关。