Abstract：
The baculovirus expression vector system using insect cells as a bioreactor has been used for in vitro expression of recombinant proteins and plays an important role in the fields of biology, agronomy, and medicine. Screening suitable host cell lines is an important part of the construction of insect cell baculovirus expression systems. In previous research, we used a single-cell cloning process with the Papilio xuthus cell line RIRI-PX1 and obtained the monoclonal cell line RIRI-PX1-C31. In this study, we compared the basic biological and recombinant protein expression characteristics of RIRI-PX1-C31 and its parent cell line RIRI-PX1 and found that the expression of recombinant β-galactosidase in RIRI-PX1-C31 was significantly higher than that in the parental cell line. Further serum-free adaptation studies confirmed that RIRI-PX1-C31 can adapt to the growth environment of Express Five Serum-free medium and that its expression level of recombinant β-galactosidase was significantly higher than that before adaptation.
摘要：
以昆虫细胞为生物反应器的杆状病毒表达载体系统已用于重组蛋白的体外表达，在生物学领域发挥着重要作用。农学，和医学。筛选合适的宿主细胞系是构建昆虫细胞杆状病毒表达系统的重要组成部分。在以前的研究中，我们使用Papilioxuthus细胞系RIRI-PX1进行单细胞克隆，并获得了单克隆细胞系RIRI-PX1-C31。在这项研究中,我们比较了RIRI-PX1-C31及其亲本细胞系RIRI-PX1的基本生物学和重组蛋白表达特征，发现RIRI-PX1-C31中重组β-半乳糖苷酶的表达明显高于亲本细胞系。进一步的无血清适应研究证实RIRI-PX1-C31能适应ExpressFive无血清培养基的生长环境,其重组β-半乳糖苷酶的表达水平明显高于适应前。
