Abstract：
The fruit nutrigenomics is an interesting and important research area towards nutrition enhancement. The phytic acid is one of the major antinutrient compound, present in seeded fruits and crops. It hinders the absorption of iron (Fe), zinc (Zn), magnesium (Mg), potassium (K) and calcium (Ca), causing mineral deficiencies. In the present study, the BsPhy gene was overexpressed in the cucumber fruits using the tomato fruit specific E8 and constitutive CaMV 35S promoter. The E8 promoter imparted heterologous expression of GUS gene in cucumber fruits, furthermore, the fruit specific expression of E8 promoter with BsPhy gene was confirmed in transgenics (E8::BsPhy) using anti rabbit-phytase antibody. The physio-biochemical analysis of transgenics revealed, maximum phytase activity in E8::BsPhy cucumber fruits at 10 days after anthesis (DAA) compared to 35S::BsPhy and wild-type (WT) fruits. Consequently, E8::BsPhy fruits also showed increased amount of inorganic phosphorus (Pi), total phosphorus (P), minerals (Zn, Fe, Mg, K, Ca), total carotenoid and other macronutrients at 10 DAA compared to 35S::BsPhy fruits. The metabolite profiling of fruits (10 DAA) showed increased sugars, amino acids, sugar acids and polyols, in both E8::BsPhy and 35S::BsPhy transgenics suggesting higher phytate metabolism, compared to WT fruits. Interestingly, both the transgenic fruits showed higher fruit biomass and yield along with improved nutritional quality, which can be attributed to increased P and Zn contents in transgenic fruits, compared to WT fruits. Our findings reveal that the BsPhy gene enhances minerals and macronutrients in transgenic cucumber fruits making it nutritious and healthy.
摘要：
水果营养基因组学是营养增强的一个有趣而重要的研究领域。植酸是主要的抗营养化合物之一，存在于种子水果和作物中。它阻碍了铁（Fe）的吸收，锌(Zn),镁(Mg),钾（K）和钙（Ca），导致矿物质缺乏。在本研究中,使用番茄果实特异性E8和组成型CaMV35S启动子在黄瓜果实中过表达Bsphy基因。E8启动子在黄瓜果实中赋予GUS基因异源表达,此外，使用抗兔植酸酶抗体在转基因（E8：：BsPhy）中证实了具有BsPhy基因的E8启动子的果实特异性表达。转基因的生理生化分析显示，与35S::Bsphy和野生型（WT）果实相比，E8::Bsphy黄瓜果实在开花后10天（DAA）的最大植酸酶活性。因此,E8：：Bsphy水果也显示出无机磷（Pi）的含量增加，总磷（P），矿物质（锌，Fe,Mg,K,Ca),与35S相比，10DAA的类胡萝卜素和其他大量营养素：：BsPhy水果。水果的代谢物分析（10DAA）显示糖增加，氨基酸,糖酸和多元醇，在E8::BsPhy和35S::BsPhy转基因中，表明植酸代谢较高，与WT水果相比。有趣的是,两种转基因水果均显示出更高的水果生物量和产量以及改善的营养品质，这可以归因于转基因水果中磷和锌含量的增加，与WT水果相比。我们的发现表明，Bsphy基因增强了转基因黄瓜果实中的矿物质和大量营养素，使其营养健康。
