PDF(Pubmed)
Abstract：
UNASSIGNED: Viral hepatitis is a widespread and serious infectious disease, and most patients with liver cirrhosis and hepatocellular carcinoma are prone to viral infections. T cell immunoglobulin-and mucin-domain-containing molecule-3 (Tim-3) is an immune checkpoint molecule that negatively regulates T cell responses, playing an extremely important role in controlling infectious diseases. However, reports about the role of serum soluble Tim-3 (sTim-3) in hepatitis virus infection are limited. Therefore, this study explored changes in sTim-3 levels in patients infected with hepatitis B virus (HBV), hepatitis C virus (HCV), and hepatitis E virus (HEV).
UNASSIGNED: This study applied high-sensitivity time-resolved fluorescence immunoassay for the detection of sTim-3 levels. A total of 205 cases of viral hepatitis infection (68 cases of HBV infection, 60 cases of HCV infection, and 77 cases of HEV virus infection) and 88 healthy controls were quantitatively determined. The changes in serum sTim-3 level and its clinical value in hepatitis virus infection were analyzed.
UNASSIGNED: Patients with HBV infection (14.00, 10.78-20.45 ng/mL), HCV infection (15.99, 11.83-27.00 ng/mL), or HEV infection (19.09, 10.85-33.93 ng/mL) had significantly higher sTim-3 levels than that in the healthy control group (7.69, 6.14-10.22 ng/mL, P < 0.0001). Patients with hepatitis and fibrosis infected with HBV (22.76, 12.82-37.53 ng/mL), HCV (33.06, 16.36-39.30 ng/mL), and HEV (28.90, 17.95-35.94 ng/mL) had significantly higher sTim-3 levels than patients with hepatitis without fibrosis (13.29, 7.75-17.28; 13.86, 11.48-18.64; 14.77, 9.79-29.79 ng/mL; P < 0.05).
UNASSIGNED: sTim-3 level was elevated in patients infected with HBV, HCV, or HEV and gradually increased in patients with either hepatitis or hepatitis with hepatic fibrosis. It has a certain role in the evaluation of the course of a disease after hepatitis virus infection.
UNASSIGNED: This study applied high-sensitivity time-resolved fluorescence immunoassay for the detection of sTim-3 levels. A total of 205 cases of viral hepatitis infection (68 cases of HBV infection, 60 cases of HCV infection, and 77 cases of HEV virus infection) and 88 healthy controls were quantitatively determined. The changes in serum sTim-3 level and its clinical value in hepatitis virus infection were analyzed.
UNASSIGNED: Patients with HBV infection (14.00, 10.78-20.45 ng/mL), HCV infection (15.99, 11.83-27.00 ng/mL), or HEV infection (19.09, 10.85-33.93 ng/mL) had significantly higher sTim-3 levels than that in the healthy control group (7.69, 6.14-10.22 ng/mL, P < 0.0001). Patients with hepatitis and fibrosis infected with HBV (22.76, 12.82-37.53 ng/mL), HCV (33.06, 16.36-39.30 ng/mL), and HEV (28.90, 17.95-35.94 ng/mL) had significantly higher sTim-3 levels than patients with hepatitis without fibrosis (13.29, 7.75-17.28; 13.86, 11.48-18.64; 14.77, 9.79-29.79 ng/mL; P < 0.05).
UNASSIGNED: sTim-3 level was elevated in patients infected with HBV, HCV, or HEV and gradually increased in patients with either hepatitis or hepatitis with hepatic fibrosis. It has a certain role in the evaluation of the course of a disease after hepatitis virus infection.
摘要：
■病毒性肝炎是一种广泛而严重的传染病，大多数肝硬化和肝细胞癌患者容易发生病毒感染。T细胞免疫球蛋白和含粘蛋白结构域的分子-3（Tim-3）是一种免疫检查点分子，可负调节T细胞反应，在控制传染病方面发挥着极其重要的作用。然而,关于血清可溶性Tim-3（sTim-3）在肝炎病毒感染中的作用的报道有限。因此,这项研究探讨了感染乙型肝炎病毒（HBV）的患者的sTim-3水平的变化，丙型肝炎病毒（HCV），戊型肝炎病毒（HEV）。
■本研究应用高灵敏度时间分辨荧光免疫测定法检测sTim-3水平。共205例病毒性肝炎感染(HBV感染68例,60例HCV感染，和77例HEV病毒感染）和88例健康对照进行了定量测定。分析血清sTim-3水平的变化及其在肝炎病毒感染中的临床价值。
■HBV感染患者（14.00，10.78-20.45ng/mL），HCV感染（15.99，11.83-27.00ng/mL），或HEV感染（19.09，10.85-33.93ng/mL）的sTim-3水平明显高于健康对照组（7.69，6.14-10.22ng/mL，P<0.0001)。肝炎和纤维化患者感染HBV（22.76，12.82-37.53ng/mL），HCV（33.06，16.36-39.30ng/mL），HEV（28.90，17.95-35.94ng/mL）的sTim-3水平明显高于无纤维化肝炎患者（13.29，7.75-17.28；13.86，11.48-18.64；14.77，9.79-29.79ng/mL；P<0.05）。
■感染HBV的患者的sTim-3水平升高，HCV,或HEV，在肝炎或肝纤维化肝炎患者中逐渐增加。对肝炎病毒沾染后的病程评价具有必定的感化。
■本研究应用高灵敏度时间分辨荧光免疫测定法检测sTim-3水平。共205例病毒性肝炎感染(HBV感染68例,60例HCV感染，和77例HEV病毒感染）和88例健康对照进行了定量测定。分析血清sTim-3水平的变化及其在肝炎病毒感染中的临床价值。
■HBV感染患者（14.00，10.78-20.45ng/mL），HCV感染（15.99，11.83-27.00ng/mL），或HEV感染（19.09，10.85-33.93ng/mL）的sTim-3水平明显高于健康对照组（7.69，6.14-10.22ng/mL，P<0.0001)。肝炎和纤维化患者感染HBV（22.76，12.82-37.53ng/mL），HCV（33.06，16.36-39.30ng/mL），HEV（28.90，17.95-35.94ng/mL）的sTim-3水平明显高于无纤维化肝炎患者（13.29，7.75-17.28；13.86，11.48-18.64；14.77，9.79-29.79ng/mL；P<0.05）。
■感染HBV的患者的sTim-3水平升高，HCV,或HEV，在肝炎或肝纤维化肝炎患者中逐渐增加。对肝炎病毒沾染后的病程评价具有必定的感化。
