PDF(Pubmed)
Abstract：
Deltacoronavirus (DCoV) is a genus of coronavirus (CoV) commonly found in avian and swine, but some DCoVs are capable of infecting humans, which causes the concern about interspecies transmission of DCoVs. Thus, monitoring the existence of DCoVs in animals near communities is of great importance for epidemic prevention. Black-headed gulls (Chroicocephalus ridibundus) are common migratory birds inhabiting in most urban and rural wetlands of Yunnan Province, China, which is a typical habitat for black-headed gulls to overwinter. Whether Yunnan black-headed gulls carry CoV has never been determined. In this study, we identified three strains of DCoVs in fecal samples of Yunnan black-headed gulls by reverse-transcriptional PCR and sequenced their whole genomes. Genomic analysis revealed that these three strains shared genomic identity of more than 99%, thus named DCoV HNU4-1, HNU4-2, and HNU4-3; their NSP12 showed high similarity of amino acid sequence to the homologs of falcon coronavirus UAE-HKU27 (HKU27), houbara coronavirus UAE-HKU28 (HKU28), and pigeon coronavirus UAE-HKU29 (HKU29). Since both HKU28 and HKU29 were found in Dubai, there might be cross-border transmission of these avian DCoVs through specific routes. Further coevolutionary analysis supported this speculation that HNU4 (or its ancestors) in black-headed gulls originated from HKU28 (or its homologous strain) in houbara, which was interspecies transmission between two different avian orders. In addition, interspecies transmission of DCoV, from houbara to falcon, pigeon and white-eye, from sparrow to common-magpie, and quail and mammal including porcine and Asian leopard cat, from munia to magpie-robin, was predicted. This is the first report of black-headed gull DCoV in Asia which was highly homolog to other avian DCoVs, and the very \"active\" host-switching events in DCoV were predicted, which provides important reference for the study of spread and transmission of DCoVs.
摘要：
Deltacronavirus（DCov）是一种冠状病毒（CoV），常见于禽类和猪中，但是一些DCoV能够感染人类，这引起了人们对DCoV种间传播的担忧。因此，监测社区附近动物中DCoV的存在对于防疫非常重要。黑头海鸥(Chroicocephalusridibundus)是生活在云南省大部分城乡湿地的常见候鸟,中国,这是黑头海鸥越冬的典型栖息地。云南黑头海鸥是否携带CoV尚未确定。在这项研究中,我们通过逆转录PCR鉴定了云南黑头海鸥粪便样品中的3株DCoV，并对其进行了全基因组测序。基因组分析显示，这三个菌株共有超过99%的基因组同一性，因此命名为DCoVHNU4-1，HNU4-2和HNU4-3;他们的NSP12显示氨基酸序列与猎鹰冠状病毒UAE-HKU27（HKU27）的同源物高度相似，胡巴拉冠状病毒阿联酋-HKU28（HKU28），和鸽子冠状病毒阿联酋-HKU29（HKU29）。由于HKU28和HKU29都是在迪拜发现的，这些禽类DCoV可能通过特定路线跨境传播。进一步的共同进化分析支持了这种推测，即黑头海鸥中的HNU4（或其祖先）起源于houbara的HKU28（或其同源菌株），这是两个不同禽类之间的种间传播。此外,DCoV的种间传播，从houbara到猎鹰,鸽子和白眼，从麻雀到普通喜,鹌鹑和哺乳动物，包括猪和亚洲豹猫，从munia到喜知更鸟,是预测的。这是亚洲黑头海鸥DCoV的第一份报告，与其他鸟类DCoV高度同源，并预测了DCoV中非常“活跃”的主机切换事件，为DCoV的传播和传播研究提供了重要的参考。
