关键词: PCR Treponema spp. dairy cattle digital dermatitis

来  源:   DOI:10.3390/pathogens11050510

Abstract:
Digital dermatitis (DD) is a highly contagious and infectious disease in cattle which has a considerable negative economic impact worldwide, and adversely affects animal welfare. Members of the genus Treponema are the only bacterial agents for which there is consistent evidence of participation in DD lesions. In Chile, DD has been described since the 1990s, but only under a clinical approach. To date, the presence of the pathogenic agent has not been confirmed in Chile by any type of confirmatory microbiological diagnosis. The aim of the present study was to detect the presence of Treponema spp. DNA in lesions consistent with DD, in Chilean dairy cattle for the first time. We provide PCR confirmation of Treponema spp. in Chilean dairy cattle affected by DD. The high rate of positive results, as well as the proportion of the main Treponema species involved, is in line with what have been described in published studies elsewhere. Future herd control plans should benefit from the molecular detection of these pathogenic bacteria associated with DD.
摘要:
数字皮炎(DD)是牛的高度传染性和传染性疾病,在全球范围内具有相当大的负面经济影响,并对动物福利产生不利影响。密螺旋体属的成员是唯一有一致证据表明参与DD病变的细菌制剂。在智利,DD自1990年代以来一直被描述,但只有在临床方法下。迄今为止,在智利,尚未通过任何类型的确认性微生物学诊断确认病原体的存在。本研究的目的是检测密螺旋体的存在。病变中的DNA与DD一致,智利奶牛第一次。我们提供密螺旋体的PCR确认。受DD影响的智利奶牛。阳性结果率高,以及所涉及的主要密螺旋体物种的比例,与其他地方已发表的研究中所描述的一致。未来的畜群控制计划应受益于这些与DD相关的病原菌的分子检测。
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