Abstract：
Introduction. Infectious keratoconjunctivitis is a significant ocular disease found in confined sheep. Little information about the aetiological agents and their antimicrobial susceptibility is available.Gap Statement. There is limited information on the aetiological agents involved in keratoconjunctivitis outbreaks in sheep.Aim. The present research aimed to determine the bacterial aetiological factors involved in an outbreak of infectious keratoconjunctivitis in confined lambs.Methodology. Ocular swabs were collected from 23 randomly selected lambs, which were classified into three groups according to the severity of the lesion: group I (N=6; no ocular involvement), group II (N=8; less severe injuries) and group III (N=9; more severe injuries). Isolation of aerobic bacteria and antimicrobial susceptibility tests were carried out. Molecular detection of Mollicutes was performed, and positive samples were tested to confirm the presence of the following species: Mycoplasma conjunctivae, Mycoplasma agalactiae and Mycoplasma mycoides subsp. capri.Results. Moraxella sp. and Mollicutes were detected in all groups, but we inferred that Moraxella sp. are only significant in the early stages of the disease. M. conjunctivae was detected in all tested groups, while M. agalactiae was detected in samples of group III only. One strain of Moraxella sp. was resistant to erythromycin and showed intermedite resistance to tetracycline.Conclusion. The presence of these species confirms their importance in the aetiology of this disease, and the low resistance profile observed in the studied farm suggested an increased cure success rate.
摘要：
Introduction.感染性角膜结膜炎是在局限的绵羊中发现的一种重要的眼部疾病。关于病原体及其抗菌敏感性的信息很少。差距声明。关于绵羊角膜结膜炎暴发的病因的信息有限。瞄准.本研究旨在确定与局限羔羊感染性角膜结膜炎爆发有关的细菌病因。方法论。从23只随机选择的羔羊身上收集眼拭子，根据病变的严重程度分为三组：I组（N=6；无眼部受累），II组（N=8；损伤程度较轻）和III组（N=9；损伤程度较重）。进行了需氧菌的分离和抗菌药物敏感性试验。进行了Mollicutes的分子检测，和阳性样品进行了测试，以确认以下物种的存在：支原体结膜，无乳支原体和支原体亚种。卡普里。结果。莫拉氏菌sp.在所有组中都检测到Mollicutes，但我们推断莫拉氏菌.只有在疾病的早期才有意义。在所有测试组中检测到结膜分枝杆菌，而仅在第III组的样品中检测到无乳分枝杆菌。一株莫拉氏菌。对红霉素具有抗性，对四环素表现出中间抗药性。结论。这些物种的存在证实了它们在这种疾病的病因中的重要性，在研究的农场中观察到的低电阻曲线表明固化成功率增加。
