METHODS: Twenty-two volunteers received subcutaneous injections of 0.1 mg and 0.01 mg glucagon, and saline on the abdomen. Blood flow was measured by a laser doppler blood flowmeter for 35 min after injections.
RESULTS: Injection of 0.1 mg glucagon resulted in a significant increase in blood flow compared with baseline blood flow for all time intervals. Significant increase was also observed after the 0.01 mg glucagon injection, except between two- and five-min post injection. The inter-individual variance was large and a third of the subjects did not show an apparent increase in local subcutaneous blood flow after the 0.1 mg glucagon injection.
CONCLUSIONS: This proof-of-concept study shows that micro-boluses of glucagon increases local subcutaneous blood flow on the abdomen of non-diabetic subjects. However, the vasodilative effect of glucagon is not observed in all subjects. The trial was not registered to protect intellectual property rights.
方法:22名志愿者皮下注射0.1mg和0.01mg胰高血糖素,和盐水在腹部。注射后35分钟,通过激光多普勒血流计测量血流量。
结果:在所有时间间隔内,注射0.1mg胰高血糖素均导致血流量与基线血流量显着增加。注射0.01mg胰高血糖素后也观察到显着增加,除了注射后两分钟到五分钟。个体间的差异很大,三分之一的受试者在注射0.1mg胰高血糖素后没有显示出局部皮下血流量的明显增加。
结论:这项概念验证研究表明,胰高血糖素微丸可增加非糖尿病受试者腹部的局部皮下血流量。然而,并非在所有受试者中都观察到胰高血糖素的血管舒张作用。该审判没有注册以保护知识产权。