PDF(Pubmed)
Abstract：
Cryopreservation of sperm is a routine technology in many livestock species, but not in swine. Frozen sperm must result in acceptable conception rates and produce 11 to 12 piglets/litter to be competitive with traditional cooled semen. The development of an extender that results in high post-thaw sperm quality and acceptable litter size requires the identification of factors that markedly affect post-thaw semen quality. The present study aims to first identify factors in boar sperm cryopreservation that significantly affect post-thaw sperm quality using an efficient, cost-effective, and relatively rapid approach. The Plackett-Burman experimental design is ideal for the screening of factors at their extreme, greatly reducing the amount of time and resources needed for a follow-up, full factorial design. Using commercial semen, a 9-factor, 12-run Plackett-Burman design was used on 10 boars split between 12 treatments. Through this method, glycerol concentration, cooling rate, antioxidant supplementation with GameteGuard (Membrane Protective Technologies, Inc. Fort Collins, CO), and straw size were identified as highly influential factors that affect post-thaw sperm quality. Extender type, starting osmolality, sodium dodecyl sulfate addition, and stepwise addition of glycerol were also influential for some but not all post-thaw sperm parameters (P < 0.05). Equilibration time in the straws before freezing was determined to have no impact on post-thaw sperm quality parameters. Using the Plackett-Burman design, it can be concluded that four of the nine factors warrant detailed investigation in full factorial experiments in the development of boar sperm cryopreservation extenders.
Freezing of sperm is a routine in many livestock species, but not in pigs, because it results in lower conception in small litter sizes. This study aims to identify factors in pig sperm freezing protocols that affect sperm quality using an efficient and relatively rapid approach. The Plackett–Burman experimental design is one such method used to rapidly screen factors and was the method used for this study. Using commercial pig semen, freezing factors were tested to determine their impact on sperm quality after freezing. Through this method, glycerol concentration (prevents cell damage), cooling rate (speed used to cool sperm to refrigerator temperature), antioxidants, and straw size (straws in which sperm are packaged for freezing) were identified as highly influential factors that affect sperm health. Extender type (the chemical base used to freeze sperm), starting osmolality (the concentration of salts and sugars in a solution), sodium dodecyl sulfate addition (detergent), and stepwise addition of glycerol (to prevent sperm damage) were also influential for some but not all sperm health measures tested. Using the Plackett–Burman design, it can be concluded that four of the nine factors warrant detailed investigation in the development of boar sperm freezing extenders.
Freezing of sperm is a routine in many livestock species, but not in pigs, because it results in lower conception in small litter sizes. This study aims to identify factors in pig sperm freezing protocols that affect sperm quality using an efficient and relatively rapid approach. The Plackett–Burman experimental design is one such method used to rapidly screen factors and was the method used for this study. Using commercial pig semen, freezing factors were tested to determine their impact on sperm quality after freezing. Through this method, glycerol concentration (prevents cell damage), cooling rate (speed used to cool sperm to refrigerator temperature), antioxidants, and straw size (straws in which sperm are packaged for freezing) were identified as highly influential factors that affect sperm health. Extender type (the chemical base used to freeze sperm), starting osmolality (the concentration of salts and sugars in a solution), sodium dodecyl sulfate addition (detergent), and stepwise addition of glycerol (to prevent sperm damage) were also influential for some but not all sperm health measures tested. Using the Plackett–Burman design, it can be concluded that four of the nine factors warrant detailed investigation in the development of boar sperm freezing extenders.
摘要：
冷冻保存精子是许多家畜物种的常规技术，但不是猪。冷冻精子必须产生可接受的受胎率，并产生11至12只仔猪/窝，以与传统的冷却精液竞争。产生高解冻后精子质量和可接受的产仔数的延伸剂的开发需要确定显着影响解冻后精液质量的因素。本研究旨在首先确定猪精子冷冻保存中显著影响解冻后精子质量的因素，成本效益高,和相对快速的方法。Plackett-Burman实验设计是筛选极端因素的理想选择，大大减少了后续行动所需的时间和资源，全因子设计。使用商业精液，9因素，12运行Plackett-Burman设计用于10只公猪，分为12种处理。通过这种方法,甘油浓度，冷却速率,抗氧化剂补充与GameteGuard（膜保护技术，公司。柯林斯堡，CO),和秸秆大小被确定为影响解冻后精子质量的高度影响因素。扩展器类型,开始渗透压,十二烷基硫酸钠添加，逐步添加甘油对部分但不是所有解冻后精子参数也有影响（P<0.05）。确定冷冻前吸管中的平衡时间对解冻后的精子质量参数没有影响。使用Plackett-Burman设计，可以得出结论，九个因素中的四个值得在猪精子冷冻保存补充剂开发的全因子实验中进行详细研究。
在许多家畜物种中，冷冻精子是常规的，但不是在猪身上，因为它会导致产仔数较小的受孕率较低。这项研究旨在使用有效且相对快速的方法来确定猪精子冷冻方案中影响精子质量的因素。Plackett-Burman实验设计是用于快速筛选因素的一种方法，并且是用于本研究的方法。使用商业猪精液，对冷冻因子进行检测,以确定其对冷冻后精子质量的影响.通过这种方法,甘油浓度（防止细胞损伤），冷却速率（用于将精子冷却至冰箱温度的速度），抗氧化剂,和稻草大小（精子包装冷冻的吸管）被确定为影响精子健康的高度影响因素。扩展器类型（用于冷冻精子的化学基础），起始渗透压（溶液中盐和糖的浓度），十二烷基硫酸钠添加（洗涤剂），和逐步添加甘油（以防止精子损伤）也影响一些但不是所有的精子健康测试措施。使用Plackett-Burman设计，可以得出结论，九个因素中的四个需要对公猪精子冷冻补充剂的发展进行详细调查。
在许多家畜物种中，冷冻精子是常规的，但不是在猪身上，因为它会导致产仔数较小的受孕率较低。这项研究旨在使用有效且相对快速的方法来确定猪精子冷冻方案中影响精子质量的因素。Plackett-Burman实验设计是用于快速筛选因素的一种方法，并且是用于本研究的方法。使用商业猪精液，对冷冻因子进行检测,以确定其对冷冻后精子质量的影响.通过这种方法,甘油浓度（防止细胞损伤），冷却速率（用于将精子冷却至冰箱温度的速度），抗氧化剂,和稻草大小（精子包装冷冻的吸管）被确定为影响精子健康的高度影响因素。扩展器类型（用于冷冻精子的化学基础），起始渗透压（溶液中盐和糖的浓度），十二烷基硫酸钠添加（洗涤剂），和逐步添加甘油（以防止精子损伤）也影响一些但不是所有的精子健康测试措施。使用Plackett-Burman设计，可以得出结论，九个因素中的四个需要对公猪精子冷冻补充剂的发展进行详细调查。
