Abstract：
Maize plant type is one of the main factors determining maize yield, and leaf angle is an important aspect of plant type. The rice Loose Plant Architecture1 (LPA1) gene and Arabidopsis AtIDD15/SHOOT GRAVITROPISM5 (SGR5) gene are related to their leaf angle. However, the homologous ZmLPA1 in maize has not been studied. In this study, the changing of leaf angle, as well as gene expression in leaves in maize mutant lpa1 and wild-type \'B73\' under different IAA concentrations were investigated. The regulation effect of IAA on the leaf angle of lpa1 was significantly stronger than that of the wild type. Transcriptome analysis showed that different exogenous IAA treatments had a common enrichment pathway-the indole alkaloid biosynthesis pathway-and among the differentially expressed genes, four genes-AUX1, AUX/IAA, ARF and SAUR-were significantly upregulated. This study revealed the regulation mechanism of ZmLPA1 gene on maize leaf angle and provided a promising gene resource for maize breeding.
摘要：
玉米株型是决定玉米产量的主要因素之一。叶片角度是植物类型的一个重要方面。水稻松散植物结构1（LPA1）基因和拟南芥AtIDD15/SHOOTGRAVITROPISM5（SGR5）基因与其叶角有关。然而,尚未研究玉米中ZmLPA1的同源性。在这项研究中,叶子角度的变化，研究了不同IAA浓度下玉米突变体lpa1和野生型\'B73\'叶片中的基因表达。IAA对lpa1叶角的调控感化明显强于野生型。转录组分析表明，不同的外源IAA处理具有共同的富集途径-吲哚生物碱生物合成途径-并且在差异表达基因中，四个基因-AUX1,AUX/IAA,ARF和SAUR-显著上调。本研究揭示了ZmLPA1基因对玉米叶角的调控机制,为玉米育种提供了有前景的基因资源。
