Abstract：
Previously, the Nine amino acid TransActivation Domain (9aaTAD) was identified in the Gal4 region 862-870 (DDVYNYLFD). Here, we identified 9aaTADs in the distal Gal4 orthologs by our prediction algorithm and found their conservation in the family. The 9aaTAD function as strong activators was demonstrated. We identified adjacent Gal4 region 871-811 (DEDTPPNPKKE) as a natural 9aaTAD inhibitory domain located at the extreme Gal4 terminus. Moreover, we identified conserved Gal4 region 172-185 (FDWSEEDDMSDGLP), which was capable to reverse the 9aaTAD inhibition. In conclusion, our results uncover the existence of the cryptic inhibitory domains, which need to be carefully implemented in all functional studies with transcription factors to avoid incorrect conclusions.
摘要：
以前,在Gal4区域862-870(DDVYNYLFD)中鉴定了9个氨基酸反式激活结构域(9aaTAD)。这里,我们通过我们的预测算法确定了远端Gal4直向同源物中的9aaTAD,并发现它们在家族中的保守性.证明了9aaTAD作为强活化剂的功能。我们将邻近的Gal4区域871-811(DEDTPPNPKKE)鉴定为位于Gal4末端的天然9aaTAD抑制结构域。此外，我们确定了保守的Gal4区域172-185（FDWSEEDDMSDGLP），能够逆转9aaTAD抑制。总之,我们的结果揭示了隐藏抑制域的存在，这需要在所有使用转录因子的功能研究中仔细实施，以避免错误的结论。
