Abstract：
This study aimed to evaluate the impact of a 12-week calorie-restricted diet and recreational sports training on gene expressions IL-15, ATROGIN-1 and MURF-1 in skeletal muscle of T2D patients.
Older adults with T2D (n = 39, 60 ± 6.0 years, BMI 33.5 ± 0.6 kg/m2) were randomly allocated to Diet+Soccer (DS), Diet+Running (DR) or Diet (D). The training sessions were moderate-to-high-intensity and performed 3 × 40 min/week for 12-weeks. Gene expression from vastus lateralis muscle obtained by qRT-PCR, dual-energy X-ray and fasting blood testing measurements were performed before and after 12-weeks. Statistical analysis adopted were two-way ANOVA and Paired t-test for gene expression, and RM-ANOVA test for the remainder variables.
Total body weight was reduced in ~4 kg representing body fat mass in all groups after 12-weeks (P < 0.05). HbA1c values decreased in all groups post-intervention. Lipids profile improved in the training groups (P < 0.05) after 12-weeks. ATROGIN-1 and MURF-1 mRNA reduced in the DS (1.084 ± 0.14 vs. 0.754 ± 1.14 and 1.175 ± 0.34 vs. 0.693 ± 0.12, respectively; P < 0.05), while IL-15 mRNA increased in the DR (1.056 ± 0.12 vs. 1.308 ± 0.13; P < 0.05) after 12-weeks intervention.
Recreational training with a moderate calorie-restricted diet can downregulates the expression of atrophy-associated myokines and increases the expression of anti-inflammatory gene IL-15.
Older adults with T2D (n = 39, 60 ± 6.0 years, BMI 33.5 ± 0.6 kg/m2) were randomly allocated to Diet+Soccer (DS), Diet+Running (DR) or Diet (D). The training sessions were moderate-to-high-intensity and performed 3 × 40 min/week for 12-weeks. Gene expression from vastus lateralis muscle obtained by qRT-PCR, dual-energy X-ray and fasting blood testing measurements were performed before and after 12-weeks. Statistical analysis adopted were two-way ANOVA and Paired t-test for gene expression, and RM-ANOVA test for the remainder variables.
Total body weight was reduced in ~4 kg representing body fat mass in all groups after 12-weeks (P < 0.05). HbA1c values decreased in all groups post-intervention. Lipids profile improved in the training groups (P < 0.05) after 12-weeks. ATROGIN-1 and MURF-1 mRNA reduced in the DS (1.084 ± 0.14 vs. 0.754 ± 1.14 and 1.175 ± 0.34 vs. 0.693 ± 0.12, respectively; P < 0.05), while IL-15 mRNA increased in the DR (1.056 ± 0.12 vs. 1.308 ± 0.13; P < 0.05) after 12-weeks intervention.
Recreational training with a moderate calorie-restricted diet can downregulates the expression of atrophy-associated myokines and increases the expression of anti-inflammatory gene IL-15.
摘要：
本研究旨在评估12周热量限制饮食和休闲运动训练对T2D患者骨骼肌中IL-15，ATROGIN-1和MURF-1基因表达的影响。
患有T2D的老年人（n=39，60±6.0岁，BMI33.5±0.6kg/m2）随机分配给饮食足球（DS），饮食+跑步（DR）或饮食（D）。训练课程为中高强度，每周3×40分钟，持续12周。通过qRT-PCR获得的股外侧肌的基因表达，在12周前后进行双能X线和空腹血液检测.统计分析采用双向方差分析和配对t检验的基因表达，和余数变量的RM-ANOVA检验。
12周后，所有组的总体重均降低约4kg，代表体脂质量（P<0.05）。干预后所有组的HbA1c值均下降。12周后，训练组的血脂状况有所改善（P<0.05）。DS中ATROGIN-1和MURF-1mRNA减少（1.084±0.14vs.0.754±1.14和1.175±0.34vs.分别为0.693±0.12；P<0.05），而IL-15mRNA在DR中增加（1.056±0.12vs.干预12周后1.308±0.13；P<0.05）。
适度限制热量饮食的休闲训练可以下调与萎缩相关的肌细胞因子的表达，并增加抗炎基因IL-15的表达。
患有T2D的老年人（n=39，60±6.0岁，BMI33.5±0.6kg/m2）随机分配给饮食足球（DS），饮食+跑步（DR）或饮食（D）。训练课程为中高强度，每周3×40分钟，持续12周。通过qRT-PCR获得的股外侧肌的基因表达，在12周前后进行双能X线和空腹血液检测.统计分析采用双向方差分析和配对t检验的基因表达，和余数变量的RM-ANOVA检验。
12周后，所有组的总体重均降低约4kg，代表体脂质量（P<0.05）。干预后所有组的HbA1c值均下降。12周后，训练组的血脂状况有所改善（P<0.05）。DS中ATROGIN-1和MURF-1mRNA减少（1.084±0.14vs.0.754±1.14和1.175±0.34vs.分别为0.693±0.12；P<0.05），而IL-15mRNA在DR中增加（1.056±0.12vs.干预12周后1.308±0.13；P<0.05）。
适度限制热量饮食的休闲训练可以下调与萎缩相关的肌细胞因子的表达，并增加抗炎基因IL-15的表达。
