A total of 413 fecal samples, 385 lung samples, 269 throat swab samples, and 183 serum samples were collected from 413 murine rodents (Rattus norvegicus, Rattus tanezumi, and Rattus rattus) captured in urban Shenzhen. Kobuviruses were detected via RT-PCR. Only fecal samples were positive, with prevalence rates of 34.9% in Rattus norvegicus and 29.4% in Rattus tanezumi. Phylogenetic analysis based on partial 3D and complete VP1 sequence regions indicated that all of the MuKV sequences obtained belonged to Aichivirus A, and were genetically closely related to other MuKVs reported in China, Hungary, and the USA. Twenty-eight full-length MuKV sequences were acquired. Phylogenetic analysis of two sequences randomly selected from the two species (SZ59 and SZ171) indicated that they shared very high nucleotide and amino acid identity with one another (94.0 and 99.3%, respectively), and comparison with human Kobuvirus revealed amino acid identity values of ~80%. Additionally, a sewage-derived sequence shared high similarity with the rat-derived sequences identified in this study, with respective nucleotide and amino acid identity values from 86.5 and 90.7% to 87.2 and 91.1%.
The results of the current study provide evidence that murine Kobuvirus is transmitted via the fecal-oral route.
总共413个粪便样本,385个肺样本,269个咽喉拭子样本,并从413只小鼠啮齿动物(Rattusnorvegicus,Rattustanezumi,和Rattusrattus)在深圳市区捕获。通过RT-PCR检测科布病毒。只有粪便样本呈阳性,褐家鼠的患病率为34.9%,白家鼠的患病率为29.4%。基于部分3D和完整VP1序列区域的系统发育分析表明,获得的所有MuKV序列均属于AichirvirusA,在基因上与中国报道的其他MuKV密切相关,匈牙利,和美国。获得了28个全长MuKV序列。从两个物种(SZ59和SZ171)中随机选择的两个序列的系统发育分析表明,它们彼此之间具有非常高的核苷酸和氨基酸同一性(94.0和99.3%,分别),与人类Kobuvirus的比较显示氨基酸同一性值为〜80%。此外,污水衍生序列与本研究中确定的大鼠衍生序列具有高度相似性,相应的核苷酸和氨基酸同一性值从86.5和90.7%到87.2和91.1%。
本研究的结果提供了鼠科布病毒通过粪-口途径传播的证据。