PDF(Pubmed)
Abstract：
Prodigiosin (Pg), a secondary metabolism produced by numerous bacterial species, is known as anticancer, antibacterial, antifungal, immunosuppressant, antioxidant, antimalarial properties. Pg has been tested for antitumor activity in many different cancer cell lines but studies in LU-1, KB cell lines, and tumor-bearing mice are still limited. In this study, Serratia marcescens QBN VTCC 910026 strain (GenBank: KX674054.1) was mutated using Ethyl Methanesulfonate (EMS) to increase the production of Pg. One strain known as EMS 5 was capable of increasing prodigiosin biosynthetic yield by 52% when compared to the wild-type strain. Red bacterial pigmented colonies containing Pg were collected from solid media, lysed with acetone, purified with toluene: ethyl acetate at a ratio of 9: 1 (v/v), and then used to evaluate the potential anticancer activity. The purity of Pg was confirmed using a high-performance liquid chromatography (HPLC) method which indicated a 98% rate. Pg chemical formula which was determined using 1H-NMR and 13C-NMR spectroscopy, confirmed as prodigiosin (Pg). Human breast cancer cell lines MCF-7, oropharyngeal cancer KB, and particularly lung cancer LU-1 in vitro were used to test the anticancer activity of purified Pg compound. It showed a strong inhibitory ability in all the cancer cell lines. Furthermore, the isolated Pg had capable of inhibiting tumor growth, the tumor volume decreased by 36.82%, after 28 days. The results indicated that the bacterial prodigiosin from variants Serratia marcescens QBN VTCC 910026 strain is an encouraging fragment suitable for therapeutic applications.
摘要：
Prodigiosin(Pg),由许多细菌物种产生的次级代谢，被称为抗癌，抗菌，抗真菌药,免疫抑制剂,抗氧化剂，抗疟疾特性。Pg已经在许多不同的癌细胞系中测试了抗肿瘤活性，但在LU-1，KB细胞系中的研究，和荷瘤小鼠仍然有限。在这项研究中,使用甲磺酸乙酯(EMS)突变粘质沙雷氏菌QBNVTCC910026菌株(GenBank:KX674054.1)以增加Pg的产生。与野生型菌株相比，一种称为EMS5的菌株能够将prodigiosin生物合成产量提高52％。从固体培养基中收集含有Pg的红色细菌色素菌落，用丙酮裂解，用甲苯：乙酸乙酯以9：1（v/v）的比例纯化，然后用于评估潜在的抗癌活性。使用高效液相色谱(HPLC)方法确认Pg的纯度,该方法表明98%的比率。使用1H-NMR和13C-NMR光谱确定的Pg化学式，确认为prodigiosin（Pg）。人乳腺癌细胞系MCF-7，口咽癌KB，特别是肺癌LU-1在体外用于测试纯化的Pg化合物的抗癌活性。它在所有癌细胞系中都显示出较强的抑制能力。此外，分离的Pg能够抑制肿瘤生长，肿瘤体积减少36.82%,28天后结果表明，来自粘质沙雷氏菌QBNVTCC910026菌株的细菌prodigiosin是适合治疗应用的令人鼓舞的片段。
