Abstract：
Liamocins and Massoia lactone have many applications. In this study, the glucose-derepressed mutant Δcrea5 in which the CREA gene was removed could produce 36.5 g/L of liamocins. Furthermore, overexpression of the MSN2 gene in the mutant Δcrea5 made the transformant M60 produce 41.4 g/L of liamocins and further overexpression of the GAL1 gene in the transformant M60 rendered the transformant G40 to produce 49.5 ± 0.4 g/L of liamocins during the 10-L fermentation while their wild type strain 9-1 made only 26.3 g/L of liamocins. The expressed transcription activators Msn2 and Gal1 were localized in the nuclei, promoting expression of the genes responsible for liamocins biosynthesis and sugar transport. Massoia lactone prepared from the produced liamocins could actively kill the spores of the pathogenic fungi from the diseased human skin by inhibiting spore germination and causing cellular necrosis of the fungal spores.
摘要：
Liamocins和马苏内酯具有许多应用。在这项研究中,去除了CREA基因的葡萄糖抑制突变体Δcrea5可以产生36.5g/L的美洲霉素。此外，MSN2基因在突变体Δcrea5中的过表达使转化体M60产生41.4g/L的liamocin，而GAL1基因在转化体M60中的进一步过表达使转化体G40产生49.5±0.4g/L的liamocin在10-L发酵期间，而其野生型菌株9-1仅产生26.3g/L的liamocin。表达的转录激活因子Msn2和Gal1定位于细胞核中，促进基因的表达，这些基因负责的成膜素的生物合成和糖运输。由产生的美洲霉素制备的马苏内酯可以通过抑制孢子萌发和引起真菌孢子的细胞坏死来主动杀死患病人皮肤上的病原真菌孢子。
