%0 Journal Article
%T Liamocin overproduction by the mutants of Aureobasidium melanogenum 9-1 for effectively killing spores of the pathogenic fungi from diseased human skin by Massoia lactone.
%A Wang Z
%A Zhang M
%A Chi Z
%A Liu GL
%A Chi ZM
%A Wang Z
%A Zhang M
%A Chi Z
%A Liu GL
%A Chi ZM
%A Wang Z
%A Zhang M
%A Chi Z
%A Liu GL
%A Chi ZM
%J World J Microbiol Biotechnol
%V 38
%N 6
%D May 2022 4
%M 35507122
%F 4.253
%R 10.1007/s11274-022-03290-9
%X Liamocins and Massoia lactone have many applications. In this study, the glucose-derepressed mutant Δcrea5 in which the CREA gene was removed could produce 36.5 g/L of liamocins. Furthermore, overexpression of the MSN2 gene in the mutant Δcrea5 made the transformant M60 produce 41.4 g/L of liamocins and further overexpression of the GAL1 gene in the transformant M60 rendered the transformant G40 to produce 49.5 ± 0.4 g/L of liamocins during the 10-L fermentation while their wild type strain 9-1 made only 26.3 g/L of liamocins. The expressed transcription activators Msn2 and Gal1 were localized in the nuclei, promoting expression of the genes responsible for liamocins biosynthesis and sugar transport. Massoia lactone prepared from the produced liamocins could actively kill the spores of the pathogenic fungi from the diseased human skin by inhibiting spore germination and causing cellular necrosis of the fungal spores.