准备工作,化学结构,和来自肉芽胞子实体的水溶性杂多糖的免疫刺激活性。
关键词: 1H-1H COSY, 1H-1H correlation spectroscopy ANOVA, Analysis of variance Akt, Protein kinase B CCK-8, Cell counting kit-8 D2O, Deuterium oxide DCFH-DA, 2′,7′-Dichlorofluorescein diacetate DEPT, Distortionless enhancement by polarization transfer DMEM, Dulbecco’s modified Eagle’s medium DPBS, Dulbecco’s phosphate-buffered saline ELISA, Enzyme-linked immunosorbent assay ERK, Extracellular signal-regulated kinase FT-IR, Fourier transform infrared spectroscopy GC-MS, Gas chromatography-mass spectrometry HMBC, Heteronuclear multiple bond correlation HPGPC, High-performance gel permeation chromatography HPLC, High performance liquid chromatography HSQC, Heteronuclear single quantum correlation Heteropolysaccharide IL-6, Interleukin-6 Immunomodulatory activity IκBα, I kappa B alpha JNK, c-Jun N-terminal kinase LPS, Lipopolysaccharides MAPKs, Mitogen-activated protein kinase MCP-1, Monocyte chemoattractant protein-1 Mw, Weight average molecular weight NF-κB, Nuclear factor-kappa B NMR, Nuclear magnetic resonance NO, Nitric oxide PI3K, Phosphatidylinositol-3-kinase PMP, 1-Phenyl-3-methyl-5-pyrazolone RIPA, Radioimmunoprecipitation assay ROS, Reactive oxygen species RT-PCR, Reverse transcription-polymerase chain reaction Structural characterization Suillus granulatus TLR2, Toll-like receptor 2 TLR4, Toll-like receptor 4 TNF-α, Tumor necrosis factor-α iNOS, Inducible nitric oxide synthase
来 源:
DOI:10.1016/j.fochx.2022.100211
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