关键词： 1H-1H COSY, 1H-1H correlation spectroscopy ANOVA, Analysis of variance Akt, Protein kinase B CCK-8, Cell counting kit-8 D2O, Deuterium oxide DCFH-DA, 2′,7′-Dichlorofluorescein diacetate DEPT, Distortionless enhancement by polarization transfer DMEM, Dulbecco’s modified Eagle’s medium DPBS, Dulbecco’s phosphate-buffered saline ELISA, Enzyme-linked immunosorbent assay ERK, Extracellular signal-regulated kinase FT-IR, Fourier transform infrared spectroscopy GC-MS, Gas chromatography-mass spectrometry HMBC, Heteronuclear multiple bond correlation HPGPC, High-performance gel permeation chromatography HPLC, High performance liquid chromatography HSQC, Heteronuclear single quantum correlation Heteropolysaccharide IL-6, Interleukin-6 Immunomodulatory activity IκBα, I kappa B alpha JNK, c-Jun N-terminal kinase LPS, Lipopolysaccharides MAPKs, Mitogen-activated protein kinase MCP-1, Monocyte chemoattractant protein-1 Mw, Weight average molecular weight NF-κB, Nuclear factor-kappa B NMR, Nuclear magnetic resonance NO, Nitric oxide PI3K, Phosphatidylinositol-3-kinase PMP, 1-Phenyl-3-methyl-5-pyrazolone RIPA, Radioimmunoprecipitation assay ROS, Reactive oxygen species RT-PCR, Reverse transcription-polymerase chain reaction Structural characterization Suillus granulatus TLR2, Toll-like receptor 2 TLR4, Toll-like receptor 4 TNF-α, Tumor necrosis factor-α iNOS, Inducible nitric oxide synthase

来  源：   DOI：10.1016/j.fochx.2022.100211   PDF(Pubmed)

Abstract：
A water-soluble heteropolysaccharide (SGP2-1) was purified from Suillus granulatus fruiting bodies by anion-exchange chromatography and gel permeation chromatography. The structural characteristics were analyzed by high-performance gel permeation chromatography, high-performance liquid chromatography, Fourier transform infrared spectroscopy, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy. The immunostimulatory activity was investigated using RAW 264.7 macrophages. Results showed that SGP2-1 with weight average molecular weight of 150.75 kDa was composed of mannose, glucose, and xylose. The backbone of SGP2-1 was mainly composed of → 4)-α-Glcp-(1→, and the terminal group α-d-Glcp → was linked to the main chain by O-6 position. SGP2-1 could significantly enhance pinocytic capacity, reactive oxygen species production, and cytokines secretion. SGP2-1 exerted immunomodulatory effects through interacting with toll-like receptor 2, and activating mitogen-activated protein kinase, phosphatidylinositol-3-kinase/protein kinase B, and nuclear factor-kappa B signaling pathways. These findings indicated that SGP2-1 could be explored as a potential immunomodulatory agent for application in functional foods.
摘要：
通过阴离子交换色谱和凝胶渗透色谱从颗粒子实体中纯化了水溶性杂多糖（SGP2-1）。通过高效凝胶渗透色谱法分析了其结构特征,高效液相色谱法，傅里叶变换红外光谱,气相色谱-质谱,核磁共振波谱.使用RAW264.7巨噬细胞研究免疫刺激活性。结果表明，重均分子量为150.75kDa的SGP2-1由甘露糖组成，葡萄糖，和木糖.SGP2-1的主链主要由→4）-α-Glcp-（1→，末端基团α-d-Glcp→通过O-6位与主链连接。SGP2-1能显著增强胞吞能力,活性氧的产生，和细胞因子分泌。SGP2-1通过与toll样受体2相互作用并激活丝裂原活化蛋白激酶发挥免疫调节作用,磷脂酰肌醇-3-激酶/蛋白激酶B,和核因子-κB信号通路。这些发现表明SGP2-1可以作为潜在的免疫调节剂用于功能性食品中。
公众号