PDF(Pubmed)
Abstract：
UNASSIGNED: The purposes of this in vitro study were to investigate whether the addition of dexamethasone can compensate for any cytotoxic effects of the amide-type local anesthetics (LA) bupivacaine and ropivacaine and whether morphine and morphine-6-glucuronide (M6G) may be a safe alternative for peritendinous application.
UNASSIGNED: Biopsies of human biceps tendons (n = 6) were dissected and cultivated. Cells were characterized by the expression for tenocyte markers, collagen I, biglycan, tenascin C, scleraxis, and RUNX via reverse transcriptase-polymerase chain reaction and immunohistochemistry. Tenocytes were incubated with bupivacaine, ropivacaine, morphine, M6G, or a saline control with and without addition of dexamethasone for 15, 60, or 240 min. Cell viability was determined by quantifying the presence of adenosine-triphosphate.
UNASSIGNED: Significant time-dependent cytotoxic effects were observed for LA after all exposure times. After 15, 60, and 240 minutes, cell viability decreased to 81.1%, 49.4% and 0% (P < .001) for bupivacaine and to 81.4%, 69.6%, and 9.3% (P < .001) for ropivacaine compared to saline control. Dexamethasone did not compensate for these cytotoxic effects. Cell viability was not affected after 15, 60-min exposures to morphine and M6G but decreased significantly (P < .001) after 240 minutes compared to saline control. However, in combination with dexamethasone, tenocyte viability was significantly increased at all times for morphine (P < .01) and at 15 and 60 minutes for M6G (P < .01).
UNASSIGNED: The results showed that amide-type LA have a time-dependent cytotoxic effect on human tenocytes in vitro, which could not be compensated for by dexamethasone, whereas morphine and M6G had no cytotoxic effects on tenocytes after 15 and 60 minutes. The addition of dexamethasone to morphine and M6G had a positive effect on viability, which increased significantly compared to the opioids.
UNASSIGNED: It is known that amide-type local anesthetics used for local joint analgesia have chondrotoxic side-effects. The combined application of morphine and dexamethasone may be a safe alternative.
UNASSIGNED: Biopsies of human biceps tendons (n = 6) were dissected and cultivated. Cells were characterized by the expression for tenocyte markers, collagen I, biglycan, tenascin C, scleraxis, and RUNX via reverse transcriptase-polymerase chain reaction and immunohistochemistry. Tenocytes were incubated with bupivacaine, ropivacaine, morphine, M6G, or a saline control with and without addition of dexamethasone for 15, 60, or 240 min. Cell viability was determined by quantifying the presence of adenosine-triphosphate.
UNASSIGNED: Significant time-dependent cytotoxic effects were observed for LA after all exposure times. After 15, 60, and 240 minutes, cell viability decreased to 81.1%, 49.4% and 0% (P < .001) for bupivacaine and to 81.4%, 69.6%, and 9.3% (P < .001) for ropivacaine compared to saline control. Dexamethasone did not compensate for these cytotoxic effects. Cell viability was not affected after 15, 60-min exposures to morphine and M6G but decreased significantly (P < .001) after 240 minutes compared to saline control. However, in combination with dexamethasone, tenocyte viability was significantly increased at all times for morphine (P < .01) and at 15 and 60 minutes for M6G (P < .01).
UNASSIGNED: The results showed that amide-type LA have a time-dependent cytotoxic effect on human tenocytes in vitro, which could not be compensated for by dexamethasone, whereas morphine and M6G had no cytotoxic effects on tenocytes after 15 and 60 minutes. The addition of dexamethasone to morphine and M6G had a positive effect on viability, which increased significantly compared to the opioids.
UNASSIGNED: It is known that amide-type local anesthetics used for local joint analgesia have chondrotoxic side-effects. The combined application of morphine and dexamethasone may be a safe alternative.
摘要：
UNASSIGNED：这项体外研究的目的是研究添加地塞米松是否可以补偿酰胺型局部麻醉药（LA）布比卡因和罗哌卡因的任何细胞毒性作用，以及吗啡和吗啡-6-葡萄糖醛酸苷（M6G）是否可能是一种安全的替代药物。
未经证实:解剖并培养人二头肌肌腱(n=6)的活检组织。细胞的特征在于肌腱细胞标志物的表达，胶原蛋白I，biglycan,生腱C,scleraxis,和RUNX通过逆转录酶-聚合酶链反应和免疫组织化学。肌腱细胞与布比卡因孵育，罗哌卡因,吗啡,M6G,或盐水对照，有和没有添加地塞米松15，60，或240分钟。通过定量三磷酸腺苷的存在来测定细胞活力。
UNASSIGNED:在所有暴露时间后,对LA观察到显著的时间依赖性细胞毒性作用。15、60和240分钟后，细胞活力下降到81.1%，布比卡因的49.4%和0%(P<.001)，81.4%，69.6%,与盐水对照相比，罗哌卡因为9.3%(P<.001)。地塞米松不能补偿这些细胞毒性作用。在暴露于吗啡和M6G15、60分钟后,与盐水对照相比,细胞活力没有受到影响,但在240分钟后显著降低(P<.001)。然而,联合地塞米松，对于吗啡(P<0.01)和对于M6G(P<0.01),在15和60分钟时,腱细胞活力显著增加。
UNASSIGNED：结果表明，酰胺型LA在体外对人肌腱细胞具有时间依赖性的细胞毒性作用，这不能用地塞米松来补偿，而吗啡和M6G在15和60分钟后对肌腱细胞没有细胞毒性作用。在吗啡和M6G中添加地塞米松对生存力有积极的影响，与阿片类药物相比显著增加。
UNASSIGNED：已知用于局部关节镇痛的酰胺型局部麻醉药具有软骨毒性副作用。联合应用吗啡和地塞米松可能是一种安全的选择。
未经证实:解剖并培养人二头肌肌腱(n=6)的活检组织。细胞的特征在于肌腱细胞标志物的表达，胶原蛋白I，biglycan,生腱C,scleraxis,和RUNX通过逆转录酶-聚合酶链反应和免疫组织化学。肌腱细胞与布比卡因孵育，罗哌卡因,吗啡,M6G,或盐水对照，有和没有添加地塞米松15，60，或240分钟。通过定量三磷酸腺苷的存在来测定细胞活力。
UNASSIGNED:在所有暴露时间后,对LA观察到显著的时间依赖性细胞毒性作用。15、60和240分钟后，细胞活力下降到81.1%，布比卡因的49.4%和0%(P<.001)，81.4%，69.6%,与盐水对照相比，罗哌卡因为9.3%(P<.001)。地塞米松不能补偿这些细胞毒性作用。在暴露于吗啡和M6G15、60分钟后,与盐水对照相比,细胞活力没有受到影响,但在240分钟后显著降低(P<.001)。然而,联合地塞米松，对于吗啡(P<0.01)和对于M6G(P<0.01),在15和60分钟时,腱细胞活力显著增加。
UNASSIGNED：结果表明，酰胺型LA在体外对人肌腱细胞具有时间依赖性的细胞毒性作用，这不能用地塞米松来补偿，而吗啡和M6G在15和60分钟后对肌腱细胞没有细胞毒性作用。在吗啡和M6G中添加地塞米松对生存力有积极的影响，与阿片类药物相比显著增加。
UNASSIGNED：已知用于局部关节镇痛的酰胺型局部麻醉药具有软骨毒性副作用。联合应用吗啡和地塞米松可能是一种安全的选择。
