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Abstract：
A general mechanism for the splicing of nuclear messenger RNA precursors in eukaryotic cells has been widely accepted. This mechanism, which generates lariat RNAs possessing a branch site, seems related to the RNA-catalyzed reactions of self-splicing introns. The splicing of nuclear messenger RNA precursors involves the formation of a multicomponent complex, the spliceosome. This splicing body contains at least three different small nuclear ribonucleoprotein particles (snRNPs), U2, U5, and U4 + U6. A complex containing precursor RNA and the U2 snRNP particle is a likely intermediate in the formation of the spliceosome.
摘要：
真核细胞中核信使RNA前体剪接的一般机制已被广泛接受。这个机制,产生具有分支位点的套索RNA，似乎与自剪接内含子的RNA催化反应有关。核信使RNA前体的剪接涉及多组分复合物的形成，剪接体.这种剪接体包含至少三种不同的小细胞核核糖核蛋白颗粒（snRNPs），U2、U5和U4+U6。包含前体RNA和U2snRNP颗粒的复合物可能是剪接体形成中的中间体。
