PDF(Pubmed)
Abstract：
Noncovalent interactions between biomolecules such as proteins and nucleic acids coordinate all cellular processes through changes in proximity. Tools that perturb these interactions are and will continue to be highly valuable for basic and translational scientific endeavors. By taking cues from natural systems, such as the adaptive immune system, we can design directed evolution platforms that can generate proteins that bind to biomolecules of interest. In recent years, the platforms used to direct the evolution of biomolecular binders have greatly expanded the range of types of interactions one can evolve. Herein, we review recent advances in methods to evolve protein-protein, protein-RNA, and protein-DNA interactions.
摘要：
生物分子如蛋白质和核酸之间的非共价相互作用通过邻近变化协调所有细胞过程。干扰这些相互作用的工具现在并将继续对基础和转化科学努力非常有价值。通过从自然系统中获取线索，比如适应性免疫系统，我们可以设计定向进化平台，可以产生与感兴趣的生物分子结合的蛋白质。近年来,用于指导生物分子结合剂进化的平台极大地扩展了人们可以进化的相互作用类型的范围。在这里，我们回顾了蛋白质-蛋白质进化方法的最新进展，蛋白质-RNA，和蛋白质-DNA相互作用。
