PDF(Pubmed)
Abstract：
UNASSIGNED: To shorten the turnaround time for blood culture (BC) analyses, a rapid method was developed for the direct identification, antimicrobial susceptibility testing (AST), and multidrug resistance testing of bacteria-positive BCs.
UNASSIGNED: The mixtures in BC bottles were treated with the multistep centrifugation method developed here and the conventional culture-based method. The bacterial sediment obtained after centrifugation was analyzed directly with MALDI-TOF MS and Vitek 2 Compact, and AST was performed directly with the Kirby-Bauer (K-B) disk diffusion, VITEK 2 Compact, and E-test methods. Extended spectrum lactamases (ESBLs) were detected with discs containing cefotaxime, cefotaxime/clavulanate, ceftazidime, and ceftazidime/clavulanate, and carbapenemase was detected with the modified carbapenem inactivation method (mCIM) and EDTA-mCIM (eCIM).
UNASSIGNED: All the results of direct testing were compared to those of the conventional methods, to evaluate the accuracy of the direct methods. The accuracies of the direct Vitek 2 Compact and MALDI-TOF MS methods were 95.5% (214/224) and 90.2% (202/224), respectively. Direct AST with K-B, Vitek 2, and E-test showed category agreement of 96.0% (2611/2721), 96.1% (2614/2721), and 97.4% (2650/2721), respectively, and the major errors and very major errors were < 2% for all three methods. In the direct determination of ESBLs, the results for cefotaxime combined with cefotaxime/clavulanate were completely consistent with those after the standard isolation method. The carbapenemase detection rate with direct mCIM and eCIM was exactly the same as that with the standard method.
UNASSIGNED: These direct procedures based on multistep centrifugation are not only highly accurate but are appropriate for clinical laboratory use because the turnaround time is shorter.
UNASSIGNED: The mixtures in BC bottles were treated with the multistep centrifugation method developed here and the conventional culture-based method. The bacterial sediment obtained after centrifugation was analyzed directly with MALDI-TOF MS and Vitek 2 Compact, and AST was performed directly with the Kirby-Bauer (K-B) disk diffusion, VITEK 2 Compact, and E-test methods. Extended spectrum lactamases (ESBLs) were detected with discs containing cefotaxime, cefotaxime/clavulanate, ceftazidime, and ceftazidime/clavulanate, and carbapenemase was detected with the modified carbapenem inactivation method (mCIM) and EDTA-mCIM (eCIM).
UNASSIGNED: All the results of direct testing were compared to those of the conventional methods, to evaluate the accuracy of the direct methods. The accuracies of the direct Vitek 2 Compact and MALDI-TOF MS methods were 95.5% (214/224) and 90.2% (202/224), respectively. Direct AST with K-B, Vitek 2, and E-test showed category agreement of 96.0% (2611/2721), 96.1% (2614/2721), and 97.4% (2650/2721), respectively, and the major errors and very major errors were < 2% for all three methods. In the direct determination of ESBLs, the results for cefotaxime combined with cefotaxime/clavulanate were completely consistent with those after the standard isolation method. The carbapenemase detection rate with direct mCIM and eCIM was exactly the same as that with the standard method.
UNASSIGNED: These direct procedures based on multistep centrifugation are not only highly accurate but are appropriate for clinical laboratory use because the turnaround time is shorter.
摘要：
未经授权：为了缩短血液培养（BC）分析的周转时间，开发了一种快速的直接鉴定方法，抗菌药物敏感性试验（AST），和细菌阳性BCs的多重耐药性检测。
UNASSIGNED：用此处开发的多步离心方法和基于常规培养的方法处理BC瓶中的混合物。用MALDI-TOFMS和Vitek2Compact直接分析离心后获得的细菌沉淀物，AST直接用Kirby-Bauer(K-B)圆盘扩散进行，VITEK2紧凑型，和电子测试方法。用含有头孢噻肟的圆盘检测出超广谱内酰胺酶（ESBLs），头孢噻肟/克拉维，头孢他啶,和头孢他啶/克拉维酸,用改良的碳青霉烯类灭活法(mCIM)和EDTA-mCIM(eCIM)检测碳青霉烯酶。
UNASSIGNED：将所有直接测试的结果与常规方法的结果进行了比较，评估直接方法的准确性。直接Vitek2Compact和MALDI-TOFMS方法的准确性分别为95.5％（214/224）和90.2％（202/224），分别。直接AST与K-B，Vitek2和E-test显示类别一致性为96.0%(2611/2721)，96.1%（2614/2721），和97.4%(2650/2721)，分别,三种方法的主要误差和非常大的误差均<2%。在直接测定ESBLs中,头孢噻肟与头孢噻肟/克拉维酸盐联用的结果与标准分离方法完全一致。直接mCIM和eCIM的碳青霉烯酶检出率与标准方法完全相同。
UNASSIGNED：这些基于多步离心的直接程序不仅精度高，而且适合临床实验室使用，因为周转时间更短。
UNASSIGNED：用此处开发的多步离心方法和基于常规培养的方法处理BC瓶中的混合物。用MALDI-TOFMS和Vitek2Compact直接分析离心后获得的细菌沉淀物，AST直接用Kirby-Bauer(K-B)圆盘扩散进行，VITEK2紧凑型，和电子测试方法。用含有头孢噻肟的圆盘检测出超广谱内酰胺酶（ESBLs），头孢噻肟/克拉维，头孢他啶,和头孢他啶/克拉维酸,用改良的碳青霉烯类灭活法(mCIM)和EDTA-mCIM(eCIM)检测碳青霉烯酶。
UNASSIGNED：将所有直接测试的结果与常规方法的结果进行了比较，评估直接方法的准确性。直接Vitek2Compact和MALDI-TOFMS方法的准确性分别为95.5％（214/224）和90.2％（202/224），分别。直接AST与K-B，Vitek2和E-test显示类别一致性为96.0%(2611/2721)，96.1%（2614/2721），和97.4%(2650/2721)，分别,三种方法的主要误差和非常大的误差均<2%。在直接测定ESBLs中,头孢噻肟与头孢噻肟/克拉维酸盐联用的结果与标准分离方法完全一致。直接mCIM和eCIM的碳青霉烯酶检出率与标准方法完全相同。
UNASSIGNED：这些基于多步离心的直接程序不仅精度高，而且适合临床实验室使用，因为周转时间更短。
