PDF(Pubmed)
Abstract：
Hymenopterans are an untapped source of venom secretions. Their recent proteo-transcriptomic studies have revealed an extraordinary pool of toxins that participate in various biological processes, including pain, paralysis, allergic reactions, and antimicrobial activities. Comprehensive and clade-specific campaigns to collect hymenopteran venoms are therefore needed. We consider that data-driven bioprospecting may help prioritise sampling and alleviate associated costs. This work established the current protein landscape from hymenopteran venoms to evaluate possible sample bias by studying their origins, sequence diversity, known structures, and biological functions. We collected all 282 reported hymenopteran toxins (peptides and proteins) from the UniProt database that we clustered into 21 protein families from the three studied clades - wasps, bees, and ants. We identified 119 biological targets of hymenopteran toxins ranging from pathogen membranes to eukaryotic proteases, ion channels and protein receptors. Our systematic study further extended to hymenopteran toxins\' therapeutic and biotechnological values, where we revealed promising applications in crop pests, human infections, autoimmune diseases, and neurodegenerative disorders.
摘要：
膜翅目是一种尚未开发的毒液分泌物来源。他们最近的蛋白质转录组学研究揭示了参与各种生物过程的毒素，包括疼痛,瘫痪过敏反应,和抗菌活性。因此，需要进行全面和特定的运动来收集膜翅目毒液。我们认为，数据驱动的生物勘探可能有助于优先考虑采样并减轻相关成本。这项工作从膜翅目毒液中建立了当前的蛋白质景观，以通过研究它们的起源来评估可能的样本偏倚，序列多样性，已知的结构，和生物学功能。我们从UniProt数据库中收集了所有282个报告的膜翅目毒素（肽和蛋白质），我们将其聚集到来自三个研究进化枝的21个蛋白质家族中-黄蜂，蜜蜂,还有蚂蚁.我们确定了119个膜翅目毒素的生物学靶标，从病原体膜到真核蛋白酶，离子通道和蛋白质受体。我们的系统研究进一步扩展到膜翅目毒素的治疗和生物技术价值，我们揭示了在作物害虫中的有希望的应用，人类感染,自身免疫性疾病，和神经退行性疾病。
