Abstract：
BACKGROUND: Legionellosis remains a public health problem. The most common diagnostic method to detect Legionella pneumophila (L. pneumophila) is culture. Polymerase chain reaction (PCR) is a fast and accurate method for this detection in environmental samples.
METHODS: Four databases were searched for studies that evaluated the detection efficiency of PCR in L. pneumophila. The quality evaluation was conducted using Review Manager 5.3. We used Meta-DiSc 1.4 software and the Stata 15.0 software to create forest plots, a meta-regression, a bivariate boxplot and a Deeks\' funnel plot.
RESULTS: A total of 18 four-fold tables from 16 studies were analysed. The overall pooled sensitivity and specificity of PCR was 94% and 72%, respectively. The positive likelihood ratio (RLR) and negative likelihood ratio (NLR) was 2.73 and 0.12, respectively. The result of the diagnostic odds ratio (DOR) was 22.85 and the area under the curve (AUC) was 0.7884.
CONCLUSIONS: Establishing a laboratory diagnostic tool for L. pneumophila detection is important for epidemiological studies. In this work, PCR demonstrated a promising diagnostic accuracy for L. pneumophila.
METHODS: Four databases were searched for studies that evaluated the detection efficiency of PCR in L. pneumophila. The quality evaluation was conducted using Review Manager 5.3. We used Meta-DiSc 1.4 software and the Stata 15.0 software to create forest plots, a meta-regression, a bivariate boxplot and a Deeks\' funnel plot.
RESULTS: A total of 18 four-fold tables from 16 studies were analysed. The overall pooled sensitivity and specificity of PCR was 94% and 72%, respectively. The positive likelihood ratio (RLR) and negative likelihood ratio (NLR) was 2.73 and 0.12, respectively. The result of the diagnostic odds ratio (DOR) was 22.85 and the area under the curve (AUC) was 0.7884.
CONCLUSIONS: Establishing a laboratory diagnostic tool for L. pneumophila detection is important for epidemiological studies. In this work, PCR demonstrated a promising diagnostic accuracy for L. pneumophila.
摘要：
背景：军团菌病仍然是一个公共卫生问题。检测嗜肺军团菌的最常见诊断方法（L.肺炎）是文化。聚合酶链反应（PCR）是一种快速，准确的检测环境样品中的方法。
方法：在4个数据库中搜索评价PCR在嗜肺乳杆菌中检测效率的研究。使用ReviewManager5.3进行质量评估。我们使用Meta-DiSc1.4软件和Stata15.0软件创建森林地块，元回归，一个双变量箱线图和一个Deeks漏斗图。
结果：分析了来自16项研究的18个4倍表格。PCR的总体汇集灵敏度和特异性分别为94%和72%，分别。阳性似然比（RLR）和阴性似然比（NLR）分别为2.73和0.12。结果诊断比值比(DOR)为22.85,曲线下面积(AUC)为0.7884。
结论：建立肺炎杆菌检测的实验室诊断工具对于流行病学研究很重要。在这项工作中,PCR显示了对嗜肺乳杆菌的有希望的诊断准确性。
方法：在4个数据库中搜索评价PCR在嗜肺乳杆菌中检测效率的研究。使用ReviewManager5.3进行质量评估。我们使用Meta-DiSc1.4软件和Stata15.0软件创建森林地块，元回归，一个双变量箱线图和一个Deeks漏斗图。
结果：分析了来自16项研究的18个4倍表格。PCR的总体汇集灵敏度和特异性分别为94%和72%，分别。阳性似然比（RLR）和阴性似然比（NLR）分别为2.73和0.12。结果诊断比值比(DOR)为22.85,曲线下面积(AUC)为0.7884。
结论：建立肺炎杆菌检测的实验室诊断工具对于流行病学研究很重要。在这项工作中,PCR显示了对嗜肺乳杆菌的有希望的诊断准确性。
