Abstract：
This systematic review (SR) with meta-analysis aimed to evaluate the scientific data related to cytogenetic damage in oral exfoliated cells of patients diagnosed with oral potentially malignant disorders (OPMDs). The SR was conducted according to the PRISMA-P guidelines. The PICOS (Participants, Intervention, Comparison, Outcome, and Study Design) strategy was used to answer the question: \"Is micronucleus assay in oral exfoliated cells a suitable biomarker for predicting cancer risk in individuals with OPMDs?\" The search strategy was performed in the following electronic databases: PubMed, Medline, Scopus and Web of Science. The comparisons were defined as standardized mean difference (SMD), and 95% confidence intervals (CI). The quality of included studies was assessed using the EPHPP (Effective Public Health Practice Project). The GRADE tool was also utilized to assess the quality of evidence of the SR. A total of 110 potentially relevant studies were selected through the search strategy. After screening titles and abstracts, 20 full-text manuscripts were assessed for eligibility and three observational studies were included in the meta-analysis. After reviewing the 20 studies, 13 were considered weak. The meta-analysis data revealed a statistically significant difference in oral micronucleated cells by patients with OPMDs when compared to control (SMD=1.77, 95% CI, 0.36-3.18, p = 0.01), with a Tau2 = 1.97; Chi2 = 66.64, and p < 0.001. Patients with OPMDs had a positive response related to mutagenicity in oral cells compared to control patients. However, SR was not able to validate the micronucleus assay as a putative biomarker in individuals with oral potentially malignant disorders since the majority of studies were considered weak based on high risk of bias.
摘要：
这项带有荟萃分析的系统评价（SR）旨在评估与诊断为口腔潜在恶性疾病（OPMDs）的患者口腔脱落细胞中细胞遗传学损伤相关的科学数据。SR是根据PRISMA-P指南进行的。PICOS（参与者，干预,比较,结果,andStudyDesign)strategywasusedtoanswerthequestion:\"IsmicrowenusassementsinoralexfoliatedcancerriskinindividualwithOPMD?\"Thesearchstrategywasperformedinthefollowingelectronicdatabase:PubMed,Medline,Scopus和WebofScience比较定义为标准化平均差(SMD)，和95%置信区间(CI)。使用EPHPP（有效公共卫生实践项目）评估纳入研究的质量。等级工具还用于评估SR的证据质量。通过搜索策略共选择了110项潜在相关研究。筛选标题和摘要后，评估了20份全文手稿的资格，并将三项观察性研究纳入荟萃分析。在回顾了20项研究后，13被认为是弱者。荟萃分析数据显示，与对照组相比，OPMD患者的口腔微核细胞差异具有统计学意义（SMD=1.77，95％CI，0.36-3.18，p=0.01），Tau2=1.97；Chi2=66.64，p<0.001。与对照组患者相比，患有OPMD的患者在口腔细胞中具有与诱变性相关的阳性反应。然而,SR无法验证微核试验作为口腔潜在恶性疾病个体的推定生物标志物，因为大多数研究基于高偏倚风险被认为是薄弱的。
