PDF(Pubmed)
Abstract：
Research into materials for medical application draws inspiration from naturally occurring or synthesized surfaces, just like many other research directions. For medical application of materials, particular attention has to be paid to biocompatibility, osseointegration, and bacterial adhesion behavior. To understand their properties and behavior, experimental studies with natural materials such as teeth are strongly required. The results, however, may be highly case-dependent because natural surfaces have the disadvantage of being subject to wide variations, for instance in their chemical composition, structure, morphology, roughness, and porosity. A synthetic surface which mimics enamel in its performance with respect to bacterial adhesion and biocompatibility would, therefore, facilitate systematic studies much better. In this study, we discuss the possibility of using hydroxyapatite (HAp) pellets to simulate the surfaces of teeth and show the possibility and limitations of using a model surface. We performed single-cell force spectroscopy with single Staphylococcus aureus cells to measure adhesion-related parameters such as adhesion force and rupture length of cell wall proteins binding to HAp and enamel. We also examine the influence of blood plasma and saliva on the adhesion properties of S. aureus. The results of these measurements are matched to water wettability, elemental composition of the samples, and the change in the macromolecules adsorbed over time on the surface. We found that the adhesion properties of S. aureus were similar on HAp and enamel samples under all conditions: Significant decreases in adhesion strength were found equally in the presence of saliva or blood plasma on both surfaces. We therefore conclude that HAp pellets are a good alternative for natural dental material. This is especially true when slight variations in the physicochemical properties of the natural materials may affect the experimental series.
摘要：
对医学应用材料的研究从天然存在或合成的表面中汲取灵感，就像许多其他研究方向一样。对于材料的医疗应用，必须特别注意生物相容性，骨整合，和细菌粘附行为。为了了解它们的性质和行为，实验研究与天然材料如牙齿是非常必要的。结果，然而,可能是高度依赖于情况，因为自然表面具有受到广泛变化的缺点，比如它们的化学成分，结构,形态学,粗糙度,和孔隙度。模拟釉质在细菌粘附和生物相容性方面的性能的合成表面将，因此,更好地促进系统研究。在这项研究中,我们讨论了使用羟基磷灰石（HAp）颗粒来模拟牙齿表面的可能性，并显示了使用模型表面的可能性和局限性。我们对单个金黄色葡萄球菌细胞进行了单细胞力光谱法，以测量与粘附相关的参数，例如与HAp和釉质结合的细胞壁蛋白的粘附力和破裂长度。我们还检查了血浆和唾液对金黄色葡萄球菌粘附特性的影响。这些测量的结果与水润湿性相匹配，样品的元素组成，以及吸附在表面上的大分子随时间的变化。我们发现在所有条件下,金黄色葡萄球菌在HAp和牙釉质样品上的粘附特性相似:在两个表面上存在唾液或血浆的情况下,同样发现粘附强度的显著降低。因此，我们得出结论，HAp颗粒是天然牙科材料的良好替代品。当天然材料的物理化学性质的轻微变化可能影响实验系列时，尤其如此。
