Abstract：
Animal models of Parkinson\'s disease are useful to evaluate new treatments and to elucidate the etiology of the disease. Hence, it is necessary to have methods that allow quantification of their effectiveness. [18 F]FDOPA-PET (FDOPA-PET) imaging is outstanding for this purpose because of its capacity to measure changes in the dopaminergic pathway noninvasively and in vivo. Nevertheless, PET acquisition and quantification is time-consuming making it necessary to find faster ways to quantify FDOPA-PET data. This study evaluated Male Wistar rats by FDOPA, before and after being partially injured with 6-OHDA unilaterally. MicroPET scans with a duration of 120 min were acquired and Patlak reference plots were created to estimate the influx constant Kc in the striatum using the full dynamic scan data. Additionally, simple striatal-to-cerebral ratios (SCR) of short static acquisitions were computed and compared with the Kc values. Good correlation (r > 0.70) was obtained between Kc and SCR, acquired between 80-120 min after FDOPA administration with frames of 10 or 20 min and both methods were able to separate the FDOPA-uptake of healthy controls from that of the PD model (SCR -28%, Kc -71%). The present study concludes that Kc and SCR can be trustfully used to discriminate partially lesioned rats from healthy controls.
摘要：
帕金森病的动物模型可用于评估新的治疗方法和阐明该病的病因。因此，有必要有可以量化其有效性的方法。[18F]FDOPA-PET（FDOPA-PET）成像在此目的方面非常出色，因为它具有无创和体内测量多巴胺能途径变化的能力。然而，PET采集和量化是耗时的,这使得必须找到更快的方法来量化FDOPA-PET数据。本研究通过FDOPA评估雄性Wistar大鼠，在单方面受到6-OHDA部分伤害之前和之后。获取持续时间为120分钟的MicroPET扫描，并创建Patlak参考图以使用完整的动态扫描数据估算纹状体中的流入常数Kc。此外,计算短静态采集的简单纹状体与脑比率(SCR),并与Kc值进行比较.Kc与SCR之间具有良好的相关性（r>0.70），在FDOPA给药后80-120分钟以10或20分钟的框架获得，两种方法都能够将健康对照的FDOPA摄取与PD模型的摄取分开（SCR-28％，Kc-71%）。本研究得出结论，Kc和SCR可以可靠地用于区分部分病变大鼠与健康对照。
