Abstract：
A novel coagulase-negative Staphylococcus strain (NTUH-S172T) was isolated from human blood culture in Taiwan with preliminary identification of Staphylococcus saprophyticus. 16S rRNA gene analysis and multilocus sequence analysis (MLSA) showed that NTUH-S172T was most closely related to Staphylococcus haemolyticus. The average nucleotide identity and digital DNA-DNA hybridization values with the whole genome sequence were <95 % and<70 % when compared to the related species. Strain NTUH-S172T could be distinguished from S. haemolyticus by urease production and from Staphylococcus borealis by nitrate reduction. In addition, the matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) spectrum of NTHU-S172T was significantly different from that of S. haemolyticus, which could be used in clinical identification. In conclusion, it is proposed that this isolate represents a novel species, named Staphylococcus taiwanensis sp. nov., with type strain NTUH-S172T (=BCRC 81315T=JCM 34726T).
摘要：
从台湾的人血培养物中分离出一种新的凝固酶阴性葡萄球菌菌株（NTUH-S172T），并初步鉴定了腐生葡萄球菌。16SrRNA基因分析和多位点序列分析（MLSA）显示，NTUH-S172T与溶血葡萄球菌关系最密切。当与相关物种相比时,与全基因组序列的平均核苷酸同一性和数字DNA-DNA杂交值<95%和<70%。菌株NTUH-S172T可以通过脲酶的产生与溶血链球菌区分开，通过硝酸盐还原与北方葡萄球菌区分开。此外,NTHU-S172T的基质辅助激光解吸电离飞行时间质谱（MALDI-TOFMS）光谱与溶血链球菌有显著差异，可用于临床鉴定。总之,有人认为这种分离物代表了一种新的物种，命名为台湾葡萄球菌。11月。,与型应变NTUH-S172T（=BCRC81315T=JCM34726T）。
