Abstract：
There are hundreds of coronaviruses, most of which circulate among animals, yet there are seven types that infect humans. Three of them can cause severe acute respiratory illness-SARS-CoV, SARS-CoV-2, and MERS-CoV. Other HCoV-229E, HCoV-OC43, HCoV-NL63, and HCoV-HKU1 usually cause only mild to moderate upper respiratory tract infections. These four coronaviruses are called seasonal, because they are continuously circulating among human population and are responsible for up to 30% of all respiratory tract infections. Genetically, these low-pathogenic types are related to SARS-CoV-2. That is why questions concerning the cross-reactivity and cross-neutralization between antibodies against different types of coronaviruses have been raised. We addressed these questions by using enzyme-linked immunosorbent assays and targeted next-generation sequencing (NGS). We established the upper respiratory infection etiology for three patients who had been vaccinated with Sputnik V and tested positive on anti-SARS-CoV-2 antibodies. The symptoms included sore throat, nasal congestion, and myalgia. Their blood serum was analyzed for anti-SARS-CoV-2 antibodies in dynamics: before vaccination, and after the first and second dose of the vaccine. After the second dose, all patients were positive for IgG antibodies against SARS-CoV-2. The targeted NGS panel sequencing data analysis showed that these patients were infected with common coronavirus HCoV-OC43. These results suggest that S protein-targeted vaccine-induced antibodies against SARS-CoV-2 are not protective against seasonal coronavirus HCoV-OC43.
摘要：
有数百种冠状病毒，其中大部分在动物之间循环，然而，有七种类型感染人类。其中三种可引起严重的急性呼吸道疾病-SARS-CoV，SARS-CoV-2和MERS-CoV。其他HCoV-229E,HCoV-OC43、HCoV-NL63和HCoV-HKU1通常仅引起轻度至中度上呼吸道感染。这四种冠状病毒被称为季节性病毒，因为它们在人群中不断循环，占所有呼吸道感染的30％。基因上,这些低致病性类型与SARS-CoV-2有关。这就是为什么提出了关于针对不同类型的冠状病毒的抗体之间的交叉反应性和交叉中和的问题。我们通过使用酶联免疫吸附测定和靶向下一代测序（NGS）解决了这些问题。我们为三名接种了SputnikV疫苗并对抗SARS-CoV-2抗体呈阳性的患者建立了上呼吸道感染的病因。症状包括喉咙痛,鼻塞,和肌痛。他们的血清在动态中分析了抗SARS-CoV-2抗体：在接种疫苗之前，在第一剂和第二剂疫苗之后。第二次给药后,所有患者的SARS-CoV-2IgG抗体均为阳性.靶向NGS面板测序数据分析显示，这些患者感染了普通冠状病毒HCoV-OC43。这些结果表明，针对SARS-CoV-2的S蛋白靶向疫苗诱导的抗体对季节性冠状病毒HCoV-OC43没有保护作用。
