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Abstract：
Metabolism comprises of two axes in order to serve homeostasis: anabolism and catabolism. Both axes are interbranched with the so-called bioenergetics aspect of metabolism. There is a plethora of analytical biochemical methods to monitor metabolites and reactions in lysates, yet there is a rising need to monitor, quantify and elucidate in real time the spatiotemporal orchestration of complex biochemical reactions in living systems and furthermore to analyze the metabolic effect of chemical compounds that are destined for the clinic. The ongoing technological burst in the field of imaging creates opportunities to establish new tools that will allow investigators to monitor dynamics of biochemical reactions and kinetics of metabolites at a resolution that ranges from subcellular organelle to whole system for some key metabolites. This article provides a mini review of available toolkits to achieve this goal but also presents a perspective on the open space that can be exploited to develop novel methodologies that will merge classic biochemistry of metabolism with advanced imaging. In other words, a perspective of \"watching metabolism in real time.\"
摘要：
代谢包括两个轴以便服务于体内平衡:合成代谢和分解代谢。两个轴都是相互分支的，具有所谓的新陈代谢的生物能量学方面。有大量的分析生化方法来监测裂解物中的代谢物和反应，然而，监测的需求日益增加，实时量化和阐明生命系统中复杂生化反应的时空编排，并进一步分析用于临床的化合物的代谢作用。成像领域正在进行的技术爆发创造了建立新工具的机会，这些工具将使研究人员能够以从亚细胞器到一些关键代谢物的整个系统的分辨率监测生化反应的动力学和代谢物的动力学。本文提供了实现这一目标的可用工具包的迷你评论，但也提出了开放空间的观点，可用于开发新的方法，将经典的代谢生物化学与先进的成像相结合。换句话说,“实时观察新陈代谢的视角。\"
