18S ribosomal DNA-based PCR test for avian and mammalian DNA identification in meat products.

基于 18S 核糖体 DNA 的 PCR 测试 ， 用于肉类产品中的禽类和哺乳动物 DNA 鉴定。

• 发表时间: Mar 2022
• 来源期刊: Vet Anim Sci
• DOI: 10.1016/j.vas.2022.100234
• PMID: 35112013
• 文章类型: Journal Article
• 作者列表: Zyrianova IM,Zaripov OG,Zyrianova IM,Zaripov OG

关键词: 18S rRNA; PCR test; PCR, Polymerase chain reaction; Phylogeny; Sausage products; Tetrapod; 18S rRNA; PCR test; PCR, Polymerase chain reaction; Phylogeny; Sausage products; Tetrapod

Abstract

This work has aimed to create a PCR test to identify avian and mammalian DNA in meat products. The test is based on phylogenetic analysis of 18S ribosomal RNA (rRNA) of four major groups of Tetrapod: Amphibia, Reptilia, Mammalia, and Aves. 18S rDNA complete coding sequences from GenBank have been used for phylogenetic analysis by the Maximum Likelihood method. The alignment of these 18S rDNA sequences has been used for PCR primers modeling. We have received the following PCR fragment for these primers: for birds - 97 base pairs (bp), and for mammals - 134 bp. The difference between them in 37 bp is sufficient for separating these fragments in standard agarose gel. We have tested this PCR to identify avian or mammalian DNA in sausage products and confirmed the suitability of this test for avian (chicken) and mammalian (sheep, cows) meat identification and meat identification in sausage products.

摘要

这项工作旨在创建PCR测试，以识别肉类产品中的禽类和哺乳动物DNA。该测试基于对四足动物的四个主要群体的18S核糖体RNA（rRNA）的系统发育分析：两栖动物，爬行动物,哺乳动物,和艾夫斯。来自GenBank的18SrDNA完整编码序列已通过最大似然方法用于系统发育分析。这些18SrDNA序列的比对已用于PCR引物建模。我们已经收到了这些引物的以下PCR片段：对于鸟类-97碱基对（bp），和哺乳动物-134bp。它们之间在37bp中的差异足以在标准琼脂糖凝胶中分离这些片段。我们已经测试了该PCR以鉴定香肠产品中的禽类或哺乳动物DNA，并确认了该测试对禽类（鸡）和哺乳动物（绵羊，牛)香肠产品中的肉类鉴定和肉类鉴定。