Abstract：
This paper develops a detailed model of human and bovine erythrocytes quantifying the dependence of total cell volume upon composition of an aqueous solution in which it is immersed. The cytoplasm is represented as an aqueous solution of hemoglobin and salt (KCl or NaCl). Model-based analysis of literature data on human erythrocytes, and of new experiments with bovine erythrocytes, leads to two findings. First, the Boyle-van\'t Hoff plot for human erythrocytes is found to be well described based on a solid volume fraction of ∼0.3 in complete agreement with desiccation experiments. The linear portion of the calculated curve turns out to be numerically indistinguishable from the commonly used ideal model parameterized with an apparent osmotically inactive volume fraction of ∼0.5. This mathematical outcome explains the longstanding perceived (but actually nonexistent) disconnect between the aforementioned fractions ∼0.3 and ∼0.5. A corollarial implication is that the actual volume fraction of osmotically nonparticipant (vicinal) water is very small (∼0.035). Second, an initial crenation of bovine erythrocytes (which occurs in classical techniques for measuring membrane permeability) is found to increase their fragility to an extent which correlates well with the crenated cell volume, and would affect the permeability determination.
摘要：
本文开发了人和牛红细胞的详细模型，该模型量化了总细胞体积对浸入其中的水溶液组成的依赖性。细胞质表示为血红蛋白和盐(KCl或NaCl)的水溶液。基于模型的人红细胞文献数据分析，以及牛红细胞的新实验，导致两个发现。首先,根据与干燥实验完全一致的固体体积分数~0.3，发现人类红细胞的Boyle-van\'tHoff图得到了很好的描述。计算曲线的线性部分在数值上与通常使用的理想模型无法区分，该模型的表观渗透非活性体积分数为0.5。这个数学结果解释了上述分数~0.3和0.5之间的长期感知（但实际上不存在）脱节。Acorolarialimplicationisthattheactualvolumefractionofsmoticallynonparticipants(vicinal)waterisverysmall(jo0.035).第二,牛红细胞的初始信用（发生在测量膜通透性的经典技术中）被发现增加了它们的脆性，其程度与细胞体积密切相关。会影响渗透率的测定。
