PDF(Pubmed)
Abstract：
BACKGROUND: Banana fruits are rich in various high-value metabolites and play a key role in the human diet. Of these components, carotenoids have attracted considerable attention due to their physiological role and human health care functions. However, the accumulation patterns of carotenoids and genome-wide analysis of gene expression during banana fruit development have not been comprehensively evaluated.
RESULTS: In the present study, an integrative analysis of metabolites and transcriptome profiles in banana fruit with three different development stages was performed. A total of 11 carotenoid compounds were identified, and most of these compounds showed markedly higher abundances in mature green and/or mature fruit than in young fruit. Results were linked to the high expression of carotenoid synthesis and regulatory genes in the middle and late stages of fruit development. Co-expression network analysis revealed that 79 differentially expressed transcription factor genes may be responsible for the regulation of LCYB (lycopene β-cyclase), a key enzyme catalyzing the biosynthesis of α- and β-carotene.
CONCLUSIONS: Collectively, the study provided new insights into the understanding of dynamic changes in carotenoid content and gene expression level during banana fruit development.
RESULTS: In the present study, an integrative analysis of metabolites and transcriptome profiles in banana fruit with three different development stages was performed. A total of 11 carotenoid compounds were identified, and most of these compounds showed markedly higher abundances in mature green and/or mature fruit than in young fruit. Results were linked to the high expression of carotenoid synthesis and regulatory genes in the middle and late stages of fruit development. Co-expression network analysis revealed that 79 differentially expressed transcription factor genes may be responsible for the regulation of LCYB (lycopene β-cyclase), a key enzyme catalyzing the biosynthesis of α- and β-carotene.
CONCLUSIONS: Collectively, the study provided new insights into the understanding of dynamic changes in carotenoid content and gene expression level during banana fruit development.
摘要：
背景：香蕉果实富含各种高价值的代谢产物，在人类饮食中起着关键作用。在这些组件中，类胡萝卜素因其生理作用和人体保健功能而备受关注。然而,香蕉果实发育过程中类胡萝卜素的积累模式和基因表达的全基因组分析尚未得到全面评估。
结果：在本研究中，对具有三个不同发育阶段的香蕉果实的代谢产物和转录组谱进行了综合分析。共鉴定出11种类胡萝卜素化合物,这些化合物中的大多数在成熟的绿色和/或成熟果实中的丰度明显高于幼果。结果与果实发育中后期类胡萝卜素合成和调节基因的高表达有关。共表达网络分析显示，79个差异表达的转录因子基因可能负责调节LCYB（番茄红素β-环化酶），催化α-和β-胡萝卜素生物合成的关键酶。
结论：总的来说，该研究为理解香蕉果实发育过程中类胡萝卜素含量和基因表达水平的动态变化提供了新的见解。
结果：在本研究中，对具有三个不同发育阶段的香蕉果实的代谢产物和转录组谱进行了综合分析。共鉴定出11种类胡萝卜素化合物,这些化合物中的大多数在成熟的绿色和/或成熟果实中的丰度明显高于幼果。结果与果实发育中后期类胡萝卜素合成和调节基因的高表达有关。共表达网络分析显示，79个差异表达的转录因子基因可能负责调节LCYB（番茄红素β-环化酶），催化α-和β-胡萝卜素生物合成的关键酶。
结论：总的来说，该研究为理解香蕉果实发育过程中类胡萝卜素含量和基因表达水平的动态变化提供了新的见解。
