关键词: CRISPR/Cas Colorimetric assay Lateral flow Loop-mediated isothermal amplification Nanoparticle

Mesh : Colorimetry / methods DNA DNA Viruses Nucleic Acid Amplification Techniques / methods Sensitivity and Specificity

来  源:   DOI:10.1016/j.jviromet.2022.114461

Abstract:
Early detection is one of the ways to deal with DNA virus widespread prevalence, and it is necessary to know new diagnostic methods and techniques. Colorimetric assays are one of the most advantageous methods in detecting viruses. These methods are based on color change, which can be seen either with the naked eye or with special devices. The aim of this study is to introduce and evaluate effective colorimetric methods based on amplification, nanoparticle, CRISPR/Cas, and Lateral flow in the diagnosis of DNA viruses and to discuss the effectiveness of each of the updated methods. Compared to the other methods, colorimetric assays are preferred for faster detection, high efficiency, cheaper cost, and high sensitivity and specificity. It is expected that the spread of these viruses can be prevented by identifying and developing new methods.
摘要:
早期检测是应对DNA病毒广泛流行的方法之一,有必要了解新的诊断方法和技术。比色测定法是检测病毒的最有利方法之一。这些方法是基于颜色变化,可以用肉眼或特殊设备看到。本研究的目的是介绍和评估基于放大的有效比色方法,纳米粒子,CRISPR/Cas,和横向流动在DNA病毒诊断中的应用,并讨论了每种更新方法的有效性。与其他方法相比,比色测定是更快检测的首选,效率高,更便宜的成本,灵敏度和特异性高。预计通过鉴定和开发新方法可以防止这些病毒的传播。
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