Abstract：
Water contamination by pathogens and diseases induced by these pathogens is a major water quality issue all over the world. Poor public health has been linked to tap water polluted with DNA harboring antibiotic resistance genes sequence. According to HSAB concept, surface modification of rubber seed shell waste with alumina (AIRSS) as novel agro-waste adsorbent creates more active surface constituents for DNA adsorption. The proximate, ultimate and EDAX analysis provides the percentage levels of ash concentration, volatile, moisture and fixed carbon content, elemental composition present in the adsorbent. The structural features of AIRSS were determined using FT-IR, SEM and XRD. In order to improve reaction conditions, the effect of pH, temperature, adsorbent amount, and reaction time is also examined. The highest percent of DNA removal (92.5%) was achieved at the optimum conditions: 2 g/L at pH 4, contact time 120 minutes as compared to the conventional methods. The DNA adsorbs onto the surface of AIRSS through physical (vander Waals force) and chemical interactions, as demonstrated by kinetics and spectroscopic analyses. Changes in enthalpy (H), free energy (G), and entropy (S) indicate that adsorption is a spontaneous and exothermic process, according to thermodynamic parameters. The results of the experiments showed that the prepared AIRSP adsorbent could be used to remove DNA from water. The efficacy of AIRSS for the removal of DNA has decreased after nine months of storage and use. Low pH and the presence of AIRSS improved DNA-AIRSS adsorption, according to our findings.
摘要：
病原体引起的水污染和由这些病原体引起的疾病是全世界的主要水质问题。公共健康状况不佳与自来水被含有抗生素抗性基因序列的DNA污染有关。根据HSAB的概念,用氧化铝（AIRSS）作为新型农业废物吸附剂对橡胶种子壳废物进行表面改性，可以为DNA吸附创造更多的活性表面成分。最接近的，最终和EDAX分析提供了灰分浓度的百分比水平，volatile,水分和固定碳含量，吸附剂中存在的元素组成。使用FT-IR确定了AIRSS的结构特征，SEM和XRD。为了改善反应条件,pH的影响，温度,吸附剂用量,和反应时间也被检查。与常规方法相比,在最佳条件下达到最高的DNA去除百分比(92.5%):2g/L,pH4,接触时间120分钟。DNA通过物理（范德华力）和化学相互作用吸附到AIRSS的表面，如动力学和光谱分析所示。焓（H）的变化，自由能（G），和熵(S)表明吸附是一个自发的放热过程，根据热力学参数。实验结果表明，制备的AIRSP吸附剂可用于去除水中的DNA。在储存和使用9个月后，AIRSS去除DNA的功效有所下降。低pH和AIRSS的存在改善了DNA-AIRSS吸附，根据我们的发现。
