Abstract：
To assay salivary anti-nuclear antibody (ANA) and its isotypes in patients with systemic lupus erythematosus (SLE) and to investigate relevant clinical associations.
Saliva samples were collected from SLE patients and assayed for salivary ANA using immunofluorescence (IF). Isotypes of salivary ANA, including IgG-ANA, IgA-ANA, and IgM-ANA, were quantified using enzyme-linked immunosorbent assay. The correlations between clinical parameters and levels of salivary ANA and isotypes were evaluated.
Salivary ANA IF intensities were significantly higher in SLE patients than in healthy controls, irrespective of SLE patient disease activity, and strongly correlated with serum ANA titers. Salivary ANA was detected in 67.14% of SLE patients and 10.00% of healthy controls (p < 0.001). Among ANA-positive samples, 80.85% exhibited a nuclear ANA pattern, and 42.55% exhibited a cytoplasmic ANA pattern. Salivary IgG-ANA, IgA-ANA, and IgM-ANA levels, as assayed by ELISA, were significantly increased in both active and less active SLE patients compared with healthy controls, and levels of each isotype were significantly correlated with serum ANA titer. Salivary IgM-ANA levels correlated with the physician global assessment (PGA), SLE disease activity index (SLEDAI), and negatively with serum C3 and C4. Salivary IgG-ANA also correlated with erythrocyte sedimentation rate (ESR), SLEDAI, and negatively with serum C3.
Salivary ANA levels correlate with serum ANA titer, and salivary IgM-ANA and IgG-ANA correlate variably with PGA, SLEDAI, ESR and complement levels. These findings underscore the potential of using salivary ANA and ANA isotypes as surrogates for serum ANA, particularly for future point-of-care applications since saliva is easier to obtain than blood.
Saliva samples were collected from SLE patients and assayed for salivary ANA using immunofluorescence (IF). Isotypes of salivary ANA, including IgG-ANA, IgA-ANA, and IgM-ANA, were quantified using enzyme-linked immunosorbent assay. The correlations between clinical parameters and levels of salivary ANA and isotypes were evaluated.
Salivary ANA IF intensities were significantly higher in SLE patients than in healthy controls, irrespective of SLE patient disease activity, and strongly correlated with serum ANA titers. Salivary ANA was detected in 67.14% of SLE patients and 10.00% of healthy controls (p < 0.001). Among ANA-positive samples, 80.85% exhibited a nuclear ANA pattern, and 42.55% exhibited a cytoplasmic ANA pattern. Salivary IgG-ANA, IgA-ANA, and IgM-ANA levels, as assayed by ELISA, were significantly increased in both active and less active SLE patients compared with healthy controls, and levels of each isotype were significantly correlated with serum ANA titer. Salivary IgM-ANA levels correlated with the physician global assessment (PGA), SLE disease activity index (SLEDAI), and negatively with serum C3 and C4. Salivary IgG-ANA also correlated with erythrocyte sedimentation rate (ESR), SLEDAI, and negatively with serum C3.
Salivary ANA levels correlate with serum ANA titer, and salivary IgM-ANA and IgG-ANA correlate variably with PGA, SLEDAI, ESR and complement levels. These findings underscore the potential of using salivary ANA and ANA isotypes as surrogates for serum ANA, particularly for future point-of-care applications since saliva is easier to obtain than blood.
摘要：
检测系统性红斑狼疮（SLE）患者唾液抗核抗体（ANA）及其同种型，并探讨其临床相关性。
从SLE患者收集唾液样品并使用免疫荧光(IF)测定唾液ANA。唾液ANA的同种型，包括IgG-ANA,IgA-ANA,和IgM-ANA,使用酶联免疫吸附测定进行定量。评估了临床参数与唾液ANA和同种型水平之间的相关性。
SLE患者的唾液ANAIF强度明显高于健康对照组，无论SLE患者的疾病活动，与血清ANA滴度密切相关。在67.14%的SLE患者和10.00%的健康对照中检测到唾液ANA(p<0.001)。在ANA阳性样本中，80.85%表现出核ANA模式，42.55%表现为胞质ANA模式。唾液IgG-ANA,IgA-ANA,和IgM-ANA水平,通过ELISA测定，与健康对照组相比，活跃和不活跃的SLE患者均显着增加，各同种型水平与血清ANA滴度显著相关。唾液IgM-ANA水平与医生全球评估(PGA)相关，SLE疾病活动指数（SLEDAI），血清C3和C4呈阴性。唾液IgG-ANA也与红细胞沉降率（ESR）相关，SLEDAI,血清C3呈阴性。
唾液ANA水平与血清ANA滴度相关，唾液IgM-ANA和IgG-ANA与PGA有不同的相关性，SLEDAI,ESR和补体水平。这些发现强调了使用唾液ANA和ANA同种型作为血清ANA替代品的潜力，特别是对于未来的护理应用，因为唾液比血液更容易获得。
从SLE患者收集唾液样品并使用免疫荧光(IF)测定唾液ANA。唾液ANA的同种型，包括IgG-ANA,IgA-ANA,和IgM-ANA,使用酶联免疫吸附测定进行定量。评估了临床参数与唾液ANA和同种型水平之间的相关性。
SLE患者的唾液ANAIF强度明显高于健康对照组，无论SLE患者的疾病活动，与血清ANA滴度密切相关。在67.14%的SLE患者和10.00%的健康对照中检测到唾液ANA(p<0.001)。在ANA阳性样本中，80.85%表现出核ANA模式，42.55%表现为胞质ANA模式。唾液IgG-ANA,IgA-ANA,和IgM-ANA水平,通过ELISA测定，与健康对照组相比，活跃和不活跃的SLE患者均显着增加，各同种型水平与血清ANA滴度显著相关。唾液IgM-ANA水平与医生全球评估(PGA)相关，SLE疾病活动指数（SLEDAI），血清C3和C4呈阴性。唾液IgG-ANA也与红细胞沉降率（ESR）相关，SLEDAI,血清C3呈阴性。
唾液ANA水平与血清ANA滴度相关，唾液IgM-ANA和IgG-ANA与PGA有不同的相关性，SLEDAI,ESR和补体水平。这些发现强调了使用唾液ANA和ANA同种型作为血清ANA替代品的潜力，特别是对于未来的护理应用，因为唾液比血液更容易获得。
