Abstract：
Human sparganosis is a food-borne parasitic disease caused by the plerocercoids of Spirometra species. Clinical diagnosis of sparganosis is crucial for effective treatment, thus it is important to identify sensitive and specific antigens of plerocercoids. The aim of the current study was to identify and characterize the immunogenic proteins of Spirometra erinaceieuropaei plerocercoids that were recognized by patient sera. Crude soluble extract of the plerocercoids were separated using 2-dimensional gel electrophoresis coupled with immunoblot and mass spectrometry analysis. Based on immunoblotting patterns and mass spectrometry results, 8 antigenic proteins were identified from the plerocercoid. Among the proteins, cysteine protease protein might be developed as an antigen for diagnosis of sparganosis.
摘要：
人裂头菌病是一种食源性寄生虫病，由Spirometra物种的plerocercoids引起。临床诊断是有效治疗的关键,因此，重要的是确定敏感的和特异性的抗原plerocercoids。本研究的目的是鉴定和表征患者血清识别的Spirometra类蛋白的免疫原性蛋白。使用2维凝胶电泳与免疫印迹和质谱分析相结合的方法分离了类角砾岩的粗可溶性提取物。根据免疫印迹模式和质谱结果，从plerocercoid中鉴定出8种抗原蛋白。在蛋白质中，半胱氨酸蛋白酶蛋白可能被开发为诊断裂头虫病的抗原。
