PDF(Pubmed)
Abstract：
This study aimed to investigate the disparity in locomotor and spatial memory deficits caused by left- or right-sided unilateral vestibular deafferentation (UVD) using a mouse model of unilateral labyrinthectomy (UL) and to examine the effects of galvanic vestibular stimulation (GVS) on the deficits over 14 days. Five experimental groups were established: the left-sided and right-sided UL (Lt.-UL and Rt.-UL) groups, left-sided and right-sided UL with bipolar GVS with the cathode on the lesion side (Lt.-GVS and Rt.-GVS) groups, and a control group with sham surgery. We assessed the locomotor and cognitive-behavioral functions using the open field (OF), Y maze, and Morris water maze (MWM) tests before (baseline) and 3, 7, and 14 days after surgical UL in each group. On postoperative day (POD) 3, locomotion and spatial working memory were more impaired in the Lt.-UL group compared with the Rt.-UL group (p < 0.01, Tamhane test). On POD 7, there was a substantial difference between the groups; the locomotion and spatial navigation of the Lt.-UL group recovered significantly more slowly compared with those of the Rt.-UL group. Although the differences in the short-term spatial cognition and motor coordination were resolved by POD 14, the long-term spatial navigation deficits assessed by the MWM were significantly worse in the Lt.-UL group compared with the Rt.-UL group. GVS intervention accelerated the vestibular compensation in both the Lt.-GVS and Rt.-GVS groups in terms of improvement of locomotion and spatial cognition. The current data imply that right- and left-sided UVD impair spatial cognition and locomotion differently and result in different compensatory patterns. Sequential bipolar GVS when the cathode (stimulating) was assigned to the lesion side accelerated recovery for UVD-induced spatial cognition, which may have implications for managing the patients with spatial cognitive impairment, especially that induced by unilateral peripheral vestibular damage on the dominant side.
摘要：
本研究旨在使用单侧迷路切除术（UL）的小鼠模型研究由左侧或右侧单侧前庭去传入（UVD）引起的运动和空间记忆缺陷的差异，并研究电前庭刺激（GVS）对缺陷的影响超过14天。建立了五个实验组：左侧和右侧UL（Lt.-UL和Rt.-UL)组，具有双极GVS的左侧和右侧UL，阴极位于病变侧（Lt.-GVS和Rt.-GVS）组，对照组进行假手术。我们使用开放场(OF)评估运动和认知行为功能，Y迷宫,和Morris水迷宫（MWM）测试之前（基线）和手术UL后3、7和14天，每组。在术后第3天（POD），Lt的运动和空间工作记忆更加受损。-UL组与Rt.-UL组(p<0.01,Tamhane检验)。在POD7上，两组之间存在实质性差异；中尉的运动和空间导航。-与Rt组相比，UL组恢复明显更慢。-UL集团。尽管POD14解决了短期空间认知和运动协调的差异，但MWM评估的长期空间导航缺陷在Lt中明显恶化。-UL组与Rt.-UL集团。GVS干预加速了两个Lt的前庭补偿。-GVS和Rt.-GVS组运动和空间认知的改善。当前数据表明，右侧和左侧UVD对空间认知和运动的损害不同，并导致不同的代偿模式。当阴极（刺激）被分配到病变侧加速恢复时，顺序双极GVS用于UVD诱导的空间认知，这可能对管理空间认知障碍患者有影响，尤其是由优势侧的单侧外周前庭损伤引起的。
