PDF(Pubmed)
Abstract：
The invention of a scanning electron microscopy (SEM) pushed the imaging methods and allowed for the observation of cell details with a high resolution. Currently, SEM appears as an extremely useful tool to analyse the morphology of biological samples. The aim of this paper is to provide a set of guidelines for using SEM to analyse morphology of prokaryotic and eukaryotic cells, taking as model cases Escherichia coli bacteria and B-35 rat neuroblastoma cells. Herein, we discuss the necessity of a careful sample preparation and provide an optimised protocol that allows to observe the details of cell ultrastructure (≥ 50 nm) with a minimum processing effort. Highlighting the versatility of morphometric descriptors, we present the most informative parameters and couple them with molecular processes. In this way, we indicate the wide range of information that can be collected through SEM imaging of biological materials that makes SEM a convenient screening method to detect cell pathology.
摘要：
扫描电子显微镜（SEM）的发明推动了成像方法，并允许以高分辨率观察细胞细节。目前,SEM似乎是分析生物样品形态的非常有用的工具。本文的目的是为使用SEM分析原核和真核细胞的形态提供一套指南，以大肠杆菌和B-35大鼠神经母细胞瘤细胞为模型。在这里，我们讨论了仔细制备样品的必要性，并提供了一个优化的方案，该方案允许以最小的处理努力观察细胞超微结构(≥50nm)的细节。突出形态计量学描述符的多功能性，我们提供了信息最丰富的参数，并将它们与分子过程耦合。这样,我们指出了可以通过生物材料的SEM成像收集的广泛信息，这使得SEM成为检测细胞病理学的便捷筛选方法。
