Abstract：
Salivary glands are branching organs which develop by bud and cleft formation to create an organ with a large surface area. The epithelium and mesenchyme signal back and forth to control this branching process, with additional cues provided by the parasympathetic nerves and blood vessels that surround the developing branches. This branching morphogenesis can be recapitulated successfully in organ culture , allowing access to the tissue to follow development and manipulate the tissue interactions, and signals. To culture glands, the filter-grid method has been widely used, allowing the development of salivary glands cultured as a whole organ, or the gland epithelium in isolation, or with the surrounding craniofacial tissue in a cranial slice. Here, we describe the methods for each approach and show the applicability of culturing glands from a wide variety of species: mouse , snake, and human. The resulting samples and data from these cultures can be employed for morphological and molecular analysis, with some examples described in this chapter, bringing valuable knowledge to our understanding of branching morphogenesis.
摘要：
唾液腺是分支器官，通过芽和裂隙形成而发育，从而形成具有大表面积的器官。上皮和间充质来回发出信号来控制这个分支过程，副交感神经和围绕发育分支的血管提供了额外的线索。这种分支形态发生可以在器官培养中成功概括，允许进入组织跟踪发育并操纵组织相互作用，和信号。培养腺体，滤波网格法得到了广泛的应用,允许唾液腺作为整个器官培养的发育，或单独的腺上皮，或者在颅骨切片中周围的颅面组织。这里,我们描述了每种方法的方法，并显示了培养来自多种物种的腺体的适用性：小鼠，蛇,和人类。从这些培养物中得到的样品和数据可用于形态学和分子分析，本章中描述的一些例子，为我们对分支形态发生的理解带来有价值的知识。
