Abstract：
Approximately 1.41 million people die annually due to tuberculosis. One of the main problems in Tuberculosis eradication is the development of resistance to various antibiotics. However, current efforts to detect resistances face challenges such as limited equipment, budget, and time. This evidence-based review investigated loop-mediated isothermal amplification, an alternative molecular diagnostic tool with promising performance and applicability in developing countries, and its use combined with Au-Nanoprobe to detect antibiotic resistance in tuberculosis. The literature search was conducted through four databases (Proquest, EBSCOhost, Scopus, and Pubmed) for useful articles on loop-mediated isothermal amplification and Au-Nanoprobe in detecting tuberculosis and tuberculosis resistance. After filtering the result with inclusion and exclusion criteria, the search produced three papers that best answer the clinical question. Loop-mediated isothermal amplification amplifies a target sequence, and Au-Nanoprobe responds to the DNA specific to the target mutant, producing an observable color change. Loop-mediated isothermal amplification and Au-Nanoprobe showed 100% sensitivity and specificity in detecting rifampicin and isoniazid resistance. Another study investigated its viability to detect tuberculosis and found 98.2% sensitivity and 88.2% specificity. Combining loop-mediated isothermal amplification and Au-Nanoprobe had a shorter time to get results and should also be relatively cheaper because it does not need a high temperature to work and requires less equipment. In conclusion, loop-mediated isothermal amplification and Au-Nanoprobe can be used as an efficient and accurate method to detect isoniazid and rifampicin-resistant tuberculosis strains. The new technology is promising for developing countries due to their high disease burden but facing several healthcare barriers.
摘要：
每年约有141万人死于结核病。根除结核病的主要问题之一是对各种抗生素的耐药性的发展。然而,当前检测电阻的努力面临挑战，如设备有限，预算,和时间。这项基于证据的综述研究了环介导的等温扩增，一种在发展中国家具有良好性能和适用性的替代分子诊断工具，并将其与Au-纳米探针联合用于检测结核病中的抗生素耐药性。文献检索是通过四个数据库(Proquest，EBSCOhost,Scopus,和Pubmed）有关环介导等温扩增和Au-纳米探针在检测结核病和结核病耐药性方面的有用文章。用纳入和排除标准过滤结果后，搜索产生了三篇论文，最好地回答了临床问题。环介导等温扩增扩增靶序列,和Au-纳米探针响应特异于目标突变体的DNA，产生可观察的颜色变化。环介导的等温扩增和Au-纳米探针在检测利福平和异烟肼抗性方面显示出100％的灵敏度和特异性。另一项研究调查了其检测结核病的可行性，发现98.2%的灵敏度和88.2%的特异性。结合环介导等温扩增和Au-Nanoprobe具有更短的时间来获得结果,并且还应该相对便宜,因为它不需要高温来工作并且需要更少的设备。总之,环介导等温扩增和Au-Nanoprobe可作为检测异烟肼和利福平耐药结核菌株的一种高效、准确的方法。这项新技术对发展中国家来说是有前途的，因为它们的疾病负担很高，但面临着一些医疗保健障碍。
