PDF(Pubmed)
Abstract：
The aim of this work was the evaluation of the physico-chemical properties of a new type of liposomes that are composed of DPPC and bioconjugates of anisic acid with phosphatidylcholine. In particular, the impact of modified anisic acid phospholipids on the thermotropic parameters of liposomes was determined, which is crucial for using them as potential carriers of active substances in cancer therapies. Their properties were determined using three biophysical methods, namely differential scanning calorimetry (DSC), steady-state fluorimetry and attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). Moreover, temperature studies of liposomes composed of DPPC and bioconjugates of anisic acid with phosphatidylcholine provided information about the phase transition, fluidity regarding chain order, hydration and dynamics. The DSC results show that the main phase transition peak for conjugates of anisic acid with phosphatidylcholine molecules was broadened and shifted to a lower temperature in a concentration- and structure-dependent manner. The ATR-FTIR results and the results of measurements conducted using fluorescent probes located at different regions in the lipid bilayer are in line with DSC. The results show that the new bioconjugates with phosphatidylcholine have a significant impact on the physico-chemical properties of a membrane and cause a decrease in the temperature of the main phase transition. The consequence of this is greater fluidity of the lipid bilayer.
摘要：
这项工作的目的是评估由DPPC和茴香酸与磷脂酰胆碱的生物缀合物组成的新型脂质体的物理化学性质。特别是,测定了改性茴香酸磷脂对脂质体热致参数的影响，这对于将它们用作癌症治疗中活性物质的潜在载体至关重要。它们的性质是用三种生物物理方法测定的，即差示扫描量热法(DSC)，稳态荧光法和衰减全反射傅里叶变换红外光谱（ATR-FTIR）。此外，由DPPC和茴香酸与磷脂酰胆碱的生物缀合物组成的脂质体的温度研究提供了有关相变的信息，关于链序的流动性，水化和动力学。DSC结果表明，茴香酸与磷脂酰胆碱分子缀合物的主要相变峰变宽，并以浓度和结构依赖性方式移至较低温度。ATR-FTIR结果和使用位于脂质双层中不同区域的荧光探针进行的测量结果与DSC一致。结果表明，与磷脂酰胆碱的新生物结合物对膜的理化性质具有显着影响，并导致主要相变温度降低。其结果是脂质双层的更大的流动性。
