Abstract：
Heat stress affects the animal production and causes serious economic losses to the husbandry. Tectonin beta-propeller repeat containing 2 (TECPR2) gene plays an important role in autophagy which may affect the temperature sensation in animals. A missense mutation (XM_024981840.1:c.3989 G > A p.Arg1330His) of the transcripts X4 in the bovine TECPR2 gene was identified. In this study, the c.3989 G > A variant in TECPR2 gene was genotyped in a total of 25 cattle breeds (520 individuals). Our results indicated that the frequency of A allele showed a decreasing pattern from southern cattle to northern cattle, while the frequency of G allele showed the opposite pattern, which was consistent with the climate distribution of China. Compared with the GG genotype, southern cattle carried more the AA and AG genotypes. Furthermore, the association results carried out that the frequencies of genotypes (GG, AG, AA) and the value of climate parameters (mean annual temperature (T), relative humidity (RH) and temperature humidity index (THI) were significantly correlated (p < 0.01). Hence, we speculated that the c.3989 G > A variant of TECPR2 gene was associated with the heat tolerance trait in Chinese cattle and the locus may be considered as a molecular marker for Chinese cattle breeding.
摘要：
热应激影响动物生产,给畜牧业造成严重的经济损失。Tectoninβ-螺旋桨重复序列2（TECPR2）基因在自噬中起重要作用，自噬可能会影响动物的温度感觉。鉴定了牛TECPR2基因中转录本X4的错义突变(XM_024981840.1:c.3989G>Ap.Arg1330His)。在这项研究中,在总共25个牛品种(520个个体)中对TECPR2基因中的c.3989G>A变体进行了基因分型。我们的结果表明，A等位基因的频率显示出从南方牛到北方牛的下降模式，而G等位基因的频率表现出相反的模式，这与中国的气候分布是一致的。与GG基因型相比，南方牛携带更多的AA和AG基因型。此外，关联结果显示，基因型的频率(GG，AG,AA)和气候参数值(年平均温度(T)，相对湿度(RH)与温度湿度指数(THI)显著相关(p<0.01)。因此，我们推测，TECPR2基因的c.3989G>A变异与中国牛的耐热性状有关，该位点可能被认为是中国牛育种的分子标记。
