PDF(Pubmed)
Abstract：
Skin is the largest organ of humans. Overexposure to ultraviolet (UV) is the primary environmental factor that causes skin damage. The compound, (-)-loliode, isolated from the brown seaweed Sargassum horneri, showed strong antioxidant and anti-inflammatory activities in in vitro and in vivo models. To further explore the potential of (-)-loliode in cosmetics, in the present study, we investigated the photoprotective effect of (-)-loliode in vitro in skin cells and in vivo in zebrafish. The results indicated that (-)-loliode significantly reduced intracellular reactive oxygen species (ROS) level, improved cell viability, and suppressed apoptosis of UVB-irradiated human keratinocytes. In addition, (-)-loliode remarkably attenuated oxidative damage, improved collagen synthesis, and inhibited matrix metalloproteinases expression in UVB-irradiated human dermal fibroblasts. Furthermore, the in vivo test demonstrated that (-)-loliode effectively and dose-dependently suppressed UVB-induced zebrafish damage displayed in decreasing the levels of ROS, nitric oxide, lipid peroxidation, and cell death in UVB-irradiated zebrafish. These results indicate that (-)-loliode possesses strong photoprotective activities and suggest (-)-loliode may an ideal ingredient in the pharmaceutical and cosmeceutical industries.
摘要：
皮肤是人类最大的器官。过度暴露于紫外线(UV)是导致皮肤损伤的主要环境因素。大院，(-)-loliode,从棕色海藻中分离出来，在体外和体内模型中显示出较强的抗氧化和抗炎活性。为了进一步探索(-)-loliode在化妆品中的潜力，在本研究中，我们研究了（-）-loliode在体外皮肤细胞和体内斑马鱼的光保护作用。结果表明，（-）-loliode显着降低细胞内活性氧（ROS）水平，提高细胞活力，并抑制UVB照射的人角质形成细胞的凋亡。此外,(-)-Loliode显著减弱氧化损伤，改善胶原蛋白合成，并抑制UVB照射的人真皮成纤维细胞中基质金属蛋白酶的表达。此外，体内测试表明，（-）-loliode有效且剂量依赖性地抑制了UVB诱导的斑马鱼损伤，表现为降低ROS水平，一氧化氮,脂质过氧化，和UVB照射斑马鱼的细胞死亡。这些结果表明（-）-loliode具有很强的光保护活性，并表明（-）-loliode可能是制药和化妆品行业的理想成分。
