Abstract：
OBJECTIVE: Cell therapies, such as stem cell suspension injection, are used to treat bronchopleural fistula. Although it is safe and effective, injected cells cannot remain within the bronchioles of the fistula due to cell leakage into the thoracic cavity. Here, we inserted a \'bio plug\' into the fistula, produced using cells and a bio-3D printer, to examine the effectiveness of bio plugs for the closure of bronchopleural fistulas, the optimal cell source and the closure mechanism.
METHODS: Bio plugs were made with mesenchymal stem (stromal) cells derived from bone marrow (MSCBM), fibroblasts and rat lung micro-vessel endothelial cells using a bio-3D printer with different cell mixing ratios. Six groups, according to the presence or absence and the type of bio plugs, were compared. The plugs were inserted into the bronchi of F344 rats. The obstruction ratio and histological and immunohistochemical findings were evaluated.
RESULTS: MSCBM+ rat lung micro-vessel endothelial cell group exhibited a higher obstruction ratio among all groups excluding the MSCBM group (P = 0.039). This group had fibrosis and CD31-positive cells and fewer CD68-positive cells than MSCBM and MSCBM+ fibroblast groups.
CONCLUSIONS: Bio plugs with mixed cells, including stem cells, contribute to bronchial closure in the current experimental setting. Endothelial cells effectively maintain the structure in this model. Although bronchial closure for bronchopleural fistula could not be described as clinical conditions were not reproduced, we collected essential data on bronchial closure; however, further experiments are warranted.
METHODS: Bio plugs were made with mesenchymal stem (stromal) cells derived from bone marrow (MSCBM), fibroblasts and rat lung micro-vessel endothelial cells using a bio-3D printer with different cell mixing ratios. Six groups, according to the presence or absence and the type of bio plugs, were compared. The plugs were inserted into the bronchi of F344 rats. The obstruction ratio and histological and immunohistochemical findings were evaluated.
RESULTS: MSCBM+ rat lung micro-vessel endothelial cell group exhibited a higher obstruction ratio among all groups excluding the MSCBM group (P = 0.039). This group had fibrosis and CD31-positive cells and fewer CD68-positive cells than MSCBM and MSCBM+ fibroblast groups.
CONCLUSIONS: Bio plugs with mixed cells, including stem cells, contribute to bronchial closure in the current experimental setting. Endothelial cells effectively maintain the structure in this model. Although bronchial closure for bronchopleural fistula could not be described as clinical conditions were not reproduced, we collected essential data on bronchial closure; however, further experiments are warranted.
摘要：
目的：细胞疗法，如干细胞悬液注射，用于治疗支气管胸膜瘘。虽然安全有效,由于细胞泄漏到胸腔，注射的细胞不能保留在瘘管的细支气管内。这里,我们在瘘管里插入了一个生物插头,使用细胞和生物3D打印机生产，为了检查生物堵塞物封闭支气管胸膜瘘的有效性，最佳细胞来源和关闭机制。
方法：用骨髓来源的间充质干细胞（基质）（MSCBM）制作生物栓，使用具有不同细胞混合比的生物3D打印机，成纤维细胞和大鼠肺微血管内皮细胞。六组,根据生物塞的存在或不存在和类型，进行了比较。将塞子插入F344大鼠的支气管中。评估阻塞率以及组织学和免疫组织化学结果。
结果：MSCBM+大鼠肺微血管内皮细胞组的阻塞率高于MSCBM组（P=0.039）。与MSCBM和MSCBM+成纤维细胞组相比，该组具有纤维化和CD31阳性细胞，而CD68阳性细胞较少。
结论：带有混合细胞的生物插头，包括干细胞,在当前的实验设置中有助于支气管闭合。内皮细胞有效地维持该模型中的结构。虽然支气管胸膜瘘的支气管闭合术不能描述为临床条件没有再现，我们收集了关于支气管闭合的基本数据；然而，进一步的实验是必要的。
方法：用骨髓来源的间充质干细胞（基质）（MSCBM）制作生物栓，使用具有不同细胞混合比的生物3D打印机，成纤维细胞和大鼠肺微血管内皮细胞。六组,根据生物塞的存在或不存在和类型，进行了比较。将塞子插入F344大鼠的支气管中。评估阻塞率以及组织学和免疫组织化学结果。
结果：MSCBM+大鼠肺微血管内皮细胞组的阻塞率高于MSCBM组（P=0.039）。与MSCBM和MSCBM+成纤维细胞组相比，该组具有纤维化和CD31阳性细胞，而CD68阳性细胞较少。
结论：带有混合细胞的生物插头，包括干细胞,在当前的实验设置中有助于支气管闭合。内皮细胞有效地维持该模型中的结构。虽然支气管胸膜瘘的支气管闭合术不能描述为临床条件没有再现，我们收集了关于支气管闭合的基本数据；然而，进一步的实验是必要的。
