Abstract：
Acanthamoeba keratitis is predominantly caused by genotype T4. We report a case of severe keratitis caused by Acanthamoeba in a 39-year-old man who had prior accidental exposure to a corrosive chemical. The patient developed central full thickness ring infiltration and epithelial defect with hypopyon that required keratoplasty. The acanthamoebae isolated from the patient exhibited thermotolerance phenotype with the capability to grow well at ambient temperature and at 42°C. Analysis of a near complete 18S rRNA gene of this isolate revealed a distinct sequence that can be unequivocally assigned to genotype T12, a rare genotype incriminated in corneal infections.
摘要：
棘阿米巴角膜炎主要由基因型T4引起。我们报告了一例由棘阿米巴引起的严重角膜炎，该病例是一名39岁的男子，该男子先前曾意外接触过腐蚀性化学物质。患者出现中央全厚度环浸润和上皮缺损，并伴有需要进行角膜移植术。从患者分离的棘阿米巴表现出耐热性表型，具有在环境温度和42°C下生长良好的能力。对该分离株的几乎完整的18SrRNA基因的分析揭示了一个独特的序列，可以明确地将其分配给基因型T12，这是一种罕见的基因型，可导致角膜感染。
