PDF(Pubmed)
Abstract：
The widespread awareness of polyunsaturated fatty acids (PUFAs) benefits for human health has increased the need for their commercial production. Two oleaginous yeast were isolated from the Mediterranean Sea fish and Red Sea fish Epinephelus aeneus and E. areolatus, respectively. These marine candidates were identified by MALDI-TOF/MS biotyper® as Lodderomyces elongisporus and Rhodotorula mucilaginosa. The effect of incubation temperature (7, 15, and 26 °C) and glucose concentration (3% and 8%) on their lipids content were investigated using Sulfo-Phospho-Vanillin (SPV) assay. Their intercellular lipids were visualized by fluorescence microscope using Nile-Red dye. L. elongisporus and R. mucilaginosa produced 20.04% and 26.79% of Linoleic acid, respectively, on normal Basal-Defatted Medium (BDM). Linoleic acid (21.4-22.7%) and α-Linolenic acid (7.5-10.8%) were produced by R. mucilaginosa and L. elongisporus, on normal BDM at 15 °C. High-Glucose BDM induced a positive effect on the total lipids production that reached its maximum of 48% and 54% by R. mucilaginosa and L. elongisporus, respectively, grown at 15 °C. Remarkably, 12.12% of long-chain 15-Docosenoic acid (C22:1) and 21.49% of Tricosanoic acid (C23:0) were detected in the FAs profile of L. elongisporus, when grown on normal BDM at 26 °C. The present study is the first one reporting the FAs profile of the Egyptian Marine L. elongisporus, and its capability to accumulate high amounts of lipids under appropriate fermentation conditions; thus, it could be considered for scaling up production.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s13205-021-03010-4.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s13205-021-03010-4.
摘要：
对多不饱和脂肪酸（PUFA）对人类健康有益的广泛认识增加了对其商业生产的需求。从地中海鱼类和红海鱼Epinephelusaeneus和E.areolatus中分离出两种含油酵母，分别。通过MALDI-TOF/MSbiotyper®将这些海洋候选物鉴定为长孢菌和粘红霉素。使用硫磷酸香兰素（SPV）测定法研究了孵育温度（7、15和26°C）和葡萄糖浓度（3％和8％）对其脂质含量的影响。使用尼罗红染料通过荧光显微镜观察它们的细胞间脂质。长孢子菌和粘胶菌产生20.04%和26.79%的亚油酸，分别,在正常基础脱脂培养基（BDM）上。亚油酸（21.4-22.7％）和α-亚麻酸（7.5-10.8％）是由粘胶菌和长孢子菌产生的，在15°C的正常BDM上。高葡萄糖BDM对总脂质产量产生积极影响，通过粘胶菌和长孢子菌达到最大值48％和54％，分别,在15°C生长值得注意的是,12.12％的长链15-二十二烯酸（C22：1）和21.49％的三香酸（C23：0）在长柄乳杆菌的FAs谱中检测到，当在26°C的正常BDM上生长时。本研究是第一个报告埃及海洋L.elongisporusFAs概况的研究，及其在适当的发酵条件下积累大量脂质的能力；因此，可以考虑扩大生产。
■在线版本包含补充材料，可在10.1007/s13205-021-03010-4获得。
■在线版本包含补充材料，可在10.1007/s13205-021-03010-4获得。
