PDF(Pubmed)
Abstract：
Single-cell technologies capture cellular heterogeneity to focus on previously poorly described subpopulations of cells. Work by our laboratory and many others has metagenomically characterised a low biomass intrauterine microbial community, alongside microbial transcripts, antigens and metabolites, but the functional importance of low biomass microbial communities in placental immuno-microenvironments is still being elucidated. Given their hypothesised role in modulating inflammation and immune ontogeny to enable tolerance of beneficial microbes while warding off pathogens, there is a need for single-cell resolution. Herein, we summarise the potential for mechanistic understanding of these and other key fundamental early developmental processes by applying single-cell approaches.
摘要：
单细胞技术捕获细胞异质性，专注于先前描述不佳的细胞亚群。我们实验室和许多其他实验室的工作在宏基因上表征了低生物量的子宫内微生物群落，除了微生物转录物，抗原和代谢物，但是胎盘免疫微环境中低生物量微生物群落的功能重要性仍在阐明中。鉴于它们在调节炎症和免疫个体发育中的假设作用，以使有益微生物耐受，同时抵御病原体，需要单细胞分辨率。在这里，我们总结了通过应用单细胞方法对这些和其他关键的基本早期发育过程进行机械理解的潜力。
