PDF(Pubmed)
Abstract：
Adipose-derived mesenchymal stem/stromal cells (ASCs) are an adult stem cell population able to self-renew and differentiate into numerous cell lineages. ASCs provide a promising future for therapeutic angiogenesis due to their ability to promote blood vessel formation. Specifically, their ability to differentiate into endothelial cells (ECs) and pericyte-like cells and to secrete angiogenesis-promoting growth factors and extracellular vesicles (EVs) makes them an ideal option in cell therapy and in regenerative medicine in conditions including tissue ischemia. In recent angiogenesis research, ASCs have often been co-cultured with an endothelial cell (EC) type in order to form mature vessel-like networks in specific culture conditions. In this review, we introduce co-culture systems and co-transplantation studies between ASCs and ECs. In co-cultures, the cells communicate via direct cell-cell contact or via paracrine signaling. Most often, ASCs are found in the perivascular niche lining the vessels, where they stabilize the vascular structures and express common pericyte surface proteins. In co-cultures, ASCs modulate endothelial cells and induce angiogenesis by promoting tube formation, partly via secretion of EVs. In vivo co-transplantation of ASCs and ECs showed improved formation of functional vessels over a single cell type transplantation. Adipose tissue as a cell source for both mesenchymal stem cells and ECs for co-transplantation serves as a prominent option for therapeutic angiogenesis and blood perfusion in vivo.
摘要：
脂肪来源的间充质干细胞/基质细胞(ASC)是能够自我更新并分化成多种细胞系的成体干细胞群。ASC由于其促进血管形成的能力而为治疗性血管生成提供了有希望的未来。具体来说,它们分化成内皮细胞(ECs)和周细胞样细胞,分泌促血管生成生长因子和细胞外囊泡(EVs)的能力,使其成为包括组织缺血在内的细胞治疗和再生医学的理想选择.在最近的血管生成研究中，ASC通常与内皮细胞(EC)类型共培养,以便在特定培养条件下形成成熟的血管样网络。在这次审查中,我们介绍了ASCs和ECs之间的共培养系统和共移植研究。在共同文化中，细胞通过直接的细胞-细胞接触或通过旁分泌信号进行通信。大多数情况下,ASC在血管周围的小生境中发现，它们稳定血管结构并表达常见的周细胞表面蛋白。在共同文化中，ASCs通过促进血管形成来调节内皮细胞和诱导血管生成,部分是通过电动汽车的分泌。与单细胞类型移植相比,ASC和EC的体内共移植显示出改善的功能性血管形成。脂肪组织作为间充质干细胞和用于共移植的EC的细胞来源是体内治疗性血管生成和血液灌注的突出选择。
