Abstract：
Biomimetics offers excellent prospects for design a novel generation of improved biomaterials. Here the controlled integration of graphene oxide (GO) derivatives with a 3D marine spongin (MS) network is explored to nanoengineer novel smart bio-based constructs for bone tissue engineering. The results point out that 3D MS surfaces can be homogeneously coated by layer-by-layer (LbL) assembly of oppositely charged polyethyleneimine (PEI) and GO. Notably, the GOPEI@MS bionanocomposites present a high structural and mechanical stability under compression tests in wet conditions (shape memory). Dynamic mechanically (2 h of sinusoidal compression cyclic interval (0.5 Hz, 0-10% strain)/14 d) stimulates GOPEI@MS seeded with osteoblast (MC3T3-E1), shows a significant improvement in bioactivity, with cell proliferation being two times higher than under static conditions. Besides, the dynamic assays show that GOPEI@MS bionanocomposites are able to act as mechanical stimulus-responsive scaffolds able to resemble physiological bone extracellular matrix (ECM) requirements by strongly triggering mineralization of the bone matrix. These results prove that the environment created by the system cell-GOPEI@MS is suitable for controlling the mechanisms regulating mechanical stimulation-induced cell proliferation for potential in vivo experimentation.
摘要：
仿生学为设计新一代改进的生物材料提供了极好的前景。在这里，探索了氧化石墨烯（GO）衍生物与3D海洋海绵（MS）网络的受控集成，以纳米工程用于骨组织工程的新型智能生物基构建体。结果表明，3DMS表面可以通过带相反电荷的聚乙烯亚胺（PEI）和GO的逐层（LbL）组装均匀涂覆。值得注意的是,GOPEI@MS生物纳米复合材料在潮湿条件下的压缩测试下呈现高的结构和机械稳定性(形状记忆)。动态机械(正弦压缩循环间隔2小时(0.5赫兹，0-10％菌株）/14d）刺激接种成骨细胞（MC3T3-E1）的GOPEI@MS，显示了生物活性的显着改善，细胞增殖比静态条件下高两倍。此外,动态分析表明，GOPEI@MS生物复合材料能够作为机械刺激响应支架，能够通过强烈触发骨基质的矿化来类似于生理骨细胞外基质（ECM）要求。这些结果证明，由系统细胞-GOPEI@MS创建的环境适合于控制调节机械刺激诱导的细胞增殖的机制，以进行潜在的体内实验。
